LncRNA H19 initiates microglial pyroptosis and neuronal death in retinal ischemia/reperfusion injury.
Animals
Apoptosis
Apoptosis Regulatory Proteins
/ genetics
Caspases
/ metabolism
Inflammasomes
/ metabolism
Male
Membrane Potential, Mitochondrial
Mice, Inbred C57BL
Mice, Knockout
MicroRNAs
/ metabolism
Microglia
/ pathology
Mitochondria
/ metabolism
Neurons
/ pathology
Pyroptosis
RNA, Long Noncoding
/ genetics
Reperfusion Injury
/ genetics
Retina
/ metabolism
Retinal Diseases
/ genetics
Retinal Ganglion Cells
/ pathology
Signal Transduction
Transcriptome
Journal
Cell death and differentiation
ISSN: 1476-5403
Titre abrégé: Cell Death Differ
Pays: England
ID NLM: 9437445
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
26
11
2018
accepted:
02
05
2019
revised:
22
04
2019
pubmed:
28
5
2019
medline:
7
4
2021
entrez:
26
5
2019
Statut:
ppublish
Résumé
Ischemia-reperfusion (I/R) is a common pathology when the blood supply to an organ was disrupted and then restored. During the reperfusion process, inflammation and tissue injury were triggered, which were mediated by immunocytes and cytokines. However, the mechanisms initiating I/R-induced inflammation and driving immunocytes activation remained largely unknown. In this study, we identified long non-coding RNA (lncRNA)-H19 as the key onset of I/R-induced inflammation. We found that I/R increased lncRNA-H19 expression to significantly promote NLRP3/6 inflammasome imbalance and resulted in microglial pyroptosis, cytokines overproduction, and neuronal death. These damages were effectively inhibited by lncRNA-H19 knockout. Specifically, lncRNA-H19 functioned via sponging miR-21 to facilitate PDCD4 expression and formed a competing endogenous RNA network (ceRNET) in ischemic cascade. LncRNA H19/miR-21/PDCD4 ceRNET can directly regulate I/R-induced sterile inflammation and neuronal lesion in vivo. We thus propose that lncRNA-H19 is a previously unknown danger signals in the molecular and immunological pathways of I/R injury, and pharmacological approaches to inhibit H19 seem likely to become treatment modalities for patients in the near future based on these mechanistic findings.
Identifiants
pubmed: 31127201
doi: 10.1038/s41418-019-0351-4
pii: 10.1038/s41418-019-0351-4
pmc: PMC7206022
doi:
Substances chimiques
Apoptosis Regulatory Proteins
0
H19 long non-coding RNA
0
Inflammasomes
0
MIRN21 microRNA, mouse
0
MicroRNAs
0
RNA, Long Noncoding
0
Caspases
EC 3.4.22.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
176-191Commentaires et corrections
Type : ErratumIn
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