MicroRNA-125a inhibits tumorigenesis by targeting Smurf1 in colorectal carcinoma.
3' Untranslated Regions
/ genetics
Animals
Carcinogenesis
/ genetics
Cell Cycle
Cell Line, Tumor
Cell Movement
/ genetics
Cell Proliferation
/ genetics
Colorectal Neoplasms
/ genetics
Gene Expression Regulation, Neoplastic
/ genetics
Humans
Male
Mice
Mice, Nude
MicroRNAs
/ genetics
Neoplasm Invasiveness
/ genetics
Signal Transduction
Ubiquitin-Protein Ligases
/ genetics
Xenograft Model Antitumor Assays
/ methods
Smurf1
colorectal carcinoma
miR-125a
tumorigenesis
Journal
FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
25
01
2019
revised:
11
04
2019
accepted:
28
05
2019
pubmed:
30
5
2019
medline:
19
2
2020
entrez:
30
5
2019
Statut:
ppublish
Résumé
Aberrant expression of microRNAs (miRNAs) may contribute to the initiation and development of multiple types of human cancer. Several miRNAs have been found to be strongly correlated with the diagnosis, progression, and prognosis of colorectal carcinoma (CRC), but the role of miR-125a in CRC remains unclear. In the present study, the function of miR-125a on the expression of Smad ubiquitin regulatory factor 1 (Smurf1) was investigated in vitro and in vivo. We verified that Smurf1 is a downstream target gene of miR-125a and is involved in miR-125a-mediated regulation of CT26 cell (colon cancer cell) proliferation and migration. Overexpression of miR-125a suppresses CT26 cell growth by inhibiting cell proliferation. Additionally, wound healing assays were performed to show that overexpression of miR-125a significantly reduced CT26 cell migration, which was reversed by overexpression of Smurf1. In vivo, miR-125a overexpression downregulated the expression of Ki67 and Smurf1, thus leading to a marked reduction in tumor growth. These results revealed that miR-125a plays a critical role in CRC by directly targeting Smurf1, a finding that may facilitate the development of improved diagnostic and therapeutic techniques for CRC.
Identifiants
pubmed: 31141316
doi: 10.1002/2211-5463.12680
pmc: PMC6609577
doi:
Substances chimiques
3' Untranslated Regions
0
MIRN125 microRNA, human
0
MicroRNAs
0
SMURF1 protein, human
EC 2.3.2.26
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1305-1314Informations de copyright
© 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
Références
J Gastrointestin Liver Dis. 2013 Sep;22(3):311-20
pubmed: 24078989
Genes Dev. 2006 Aug 15;20(16):2202-7
pubmed: 16882971
Cancer Res. 2008 Dec 15;68(24):10094-104
pubmed: 19074875
N Engl J Med. 2012 Jun 21;366(25):2345-57
pubmed: 22612596
Cancer Res. 2007 Jul 1;67(13):6092-9
pubmed: 17616664
Cancer Res. 2008 Aug 1;68(15):6416-24
pubmed: 18676867
N Engl J Med. 2012 Feb 23;366(8):687-96
pubmed: 22356322
J Biol Chem. 2004 Mar 26;279(13):12854-9
pubmed: 14701828
Cancer Res. 2005 Jul 15;65(14):6029-33
pubmed: 16024602
Clin Cancer Res. 2008 Dec 15;14(24):8080-6
pubmed: 19088023
Cancer Res. 2005 Aug 15;65(16):7065-70
pubmed: 16103053
J Am Soc Nephrol. 2017 Jun;28(6):1686-1696
pubmed: 28232619
Oncotarget. 2015 Sep 22;6(28):25266-80
pubmed: 26389681
Mol Med Rep. 2016 Jun;13(6):4859-64
pubmed: 27081863
Acta Biochim Pol. 2012;59(4):467-74
pubmed: 23173124
Int J Oncol. 2011 May;38(5):1437-43
pubmed: 21399871
Am J Gastroenterol. 2008 Jun;103(6):1541-9
pubmed: 18479499
Genes Dev. 2009 Apr 1;23(7):862-76
pubmed: 19293287
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15819-24
pubmed: 19721002
Mol Cancer Res. 2003 Oct;1(12):882-91
pubmed: 14573789
Brain Pathol. 2008 Jan;18(1):122-9
pubmed: 18226107
Diabetes. 2011 Jul;60(7):1832-7
pubmed: 21709278
Front Oncol. 2018 Aug 02;8:295
pubmed: 30116722
RNA. 2008 Nov;14(11):2348-60
pubmed: 18812439
Gastroenterology. 2010 Jun;138(6):2177-90
pubmed: 20420954
Gastrointest Cancer Res. 2008 Jul;2(4 Suppl):S10-5
pubmed: 19343140
Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19983-8
pubmed: 18056640
Oncogene. 2009 Mar 12;28(10):1385-92
pubmed: 19137007
Cancer Sci. 2008 Feb;99(2):280-6
pubmed: 18201269
PLoS One. 2012;7(6):e40169
pubmed: 22768249
Int J Med Sci. 2008 Apr 03;5(2):73-9
pubmed: 18392144
Cell Mol Life Sci. 2007 Oct;64(19-20):2575-89
pubmed: 17628742
Nat Rev Genet. 2004 Jul;5(7):522-31
pubmed: 15211354
PLoS One. 2011;6(8):e23924
pubmed: 21887346
Nat Cell Biol. 2008 Feb;10(2):202-10
pubmed: 18193036
Clin Colorectal Cancer. 2016 Sep;15(3):195-203
pubmed: 26964802
Nat Rev Cancer. 2006 Nov;6(11):857-66
pubmed: 17060945
PLoS One. 2012;7(11):e50966
pubmed: 23226435
Ann N Y Acad Sci. 2015 Sep;1353:72-88
pubmed: 25877817
J Biol Chem. 2003 Oct 3;278(40):39029-36
pubmed: 12871975
Saudi Med J. 2012 Jan;33(1):24-9
pubmed: 22273643
CA Cancer J Clin. 2013 Jan;63(1):11-30
pubmed: 23335087