In-Syringe Electrokinetic Ampholytes Focusing Coupled with Electrospray Ionization Mass Spectrometry.

Amino Acid Metabolism, Inborn Errors / diagnosis Buffers Histidine / urine Histidine Ammonia-Lyase / deficiency Humans Isoelectric Focusing / methods Reproducibility of Results Spectrometry, Mass, Electrospray Ionization / methods Syringes

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
02 07 2019
Historique:
pubmed: 30 5 2019
medline: 20 9 2020
entrez: 30 5 2019
Statut: ppublish

Résumé

A 25 μL analytical glass syringe has been used for isoelectric focusing (IEF) utilizing the stainless-steel needle and plunger as electrodes. The generation of protons and hydroxyl ions at the electrodes facilitated a neutralization reaction boundary (NRB) mechanism to focus different amphoteric compounds, such as hemoglobin, bovine serum albumin, R-phycoerythrin, and histidine, within minutes. After optimization of different experimental parameters affecting the IEF process and the coupling of the IEF syringe with electrospray ionization mass spectrometry (ESI-MS), a BGE composed of NH

Identifiants

DOI: 10.1021/acs.analchem.9b00942 PMID: 31141347
pubmed: 31141347
doi: 10.1021/acs.analchem.9b00942
doi:

Substances chimiques

Buffers 0
Histidine 4QD397987E
Histidine Ammonia-Lyase EC 4.3.1.3

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

8259-8266

Auteurs

Ibraam E Mikhail (IE)

ARC Training Centre for Portable Analytical Separation Technologies (ASTech).
Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences (Chemistry) , University of Tasmania , Private Bag 75 , Hobart , Tasmania 7001 , Australia.
Department of Analytical Chemistry, Faculty of Pharmacy , Mansoura University , Mansoura City 35516 , Egypt.

Masoomeh Tehranirokh (M)

ARC Training Centre for Portable Analytical Separation Technologies (ASTech).
Trajan Scientific and Medical , Ringwood , Victoria 3134 , Australia.

Andrew A Gooley (AA)

ARC Training Centre for Portable Analytical Separation Technologies (ASTech).
Trajan Scientific and Medical , Ringwood , Victoria 3134 , Australia.

Rosanne M Guijt (RM)

ARC Training Centre for Portable Analytical Separation Technologies (ASTech).
Deakin University , Centre for Regional and Rural Futures , Geelong , Victoria 3220 , Australia.

Michael C Breadmore (MC)

ARC Training Centre for Portable Analytical Separation Technologies (ASTech).
Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences (Chemistry) , University of Tasmania , Private Bag 75 , Hobart , Tasmania 7001 , Australia.
ORCID: 0000-0001-5591-4326

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Classifications MeSH

questionsmedicales.fr › Maladies métaboliques et nutritionnelles › Maladies métaboliques › Erreurs innées du métabolisme › Aminoacidopathies congénitales › In-Syringe Electrokinetic Ampholytes Focusing...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Substances tampon › In-Syringe Electrokinetic Ampholytes Focusing...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés essentiels › Histidine › In-Syringe Electrokinetic Ampholytes Focusing...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-nitrogen lyases › Ammonia-lyases › Histidine ammonia-lyase › In-Syringe Electrokinetic Ampholytes Focusing...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › In-Syringe Electrokinetic Ampholytes Focusing...
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Focalisation isoélectrique › In-Syringe Electrokinetic Ampholytes Focusing...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › In-Syringe Electrokinetic Ampholytes Focusing...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse ESI › In-Syringe Electrokinetic Ampholytes Focusing...
questionsmedicales.fr › Équipement et fournitures › Seringues › In-Syringe Electrokinetic Ampholytes Focusing...