Peyer's patches contain abundant isotype-switched B cells with activated phenotypes and are inductive sites for T-independent anti-DNA IgA.
Animals
Antibodies, Antinuclear
/ metabolism
B-Lymphocytes
/ immunology
Cells, Cultured
Female
Gastrointestinal Microbiome
/ immunology
Hybridomas
Immunoglobulin A
/ metabolism
Immunoglobulin Class Switching
Intestines
/ immunology
Lymphocyte Activation
Male
Mice
Mice, Inbred C57BL
Peyer's Patches
/ immunology
Phenotype
T-Lymphocytes
/ immunology
B cells
IgA
Peyer’s patch
Journal
Immunology letters
ISSN: 1879-0542
Titre abrégé: Immunol Lett
Pays: Netherlands
ID NLM: 7910006
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
07
05
2019
revised:
21
05
2019
accepted:
26
05
2019
pubmed:
31
5
2019
medline:
4
4
2020
entrez:
31
5
2019
Statut:
ppublish
Résumé
Peyer's patches (PPs) are inductive sites for IgA production; however, the induction mechanism of IgA remains largely unknown. We found that the activated phenotypes of isotype-switched PP B cells were more abundant than those of splenic B cells. Immunoglobulins (Igs) from PP B cells reacted to several substances, including DNA and diet extract. Hybridomas established from PP B cells of untreated mice revealed that IgA mainly react with DNA. PP-deficient mice revealed that PPs were dispensable for a total intestinal IgA amount but were required for intestinal anti-diet extract and anti-DNA IgA. Antibiotic-treated mice and CD4
Identifiants
pubmed: 31145915
pii: S0165-2478(19)30243-3
doi: 10.1016/j.imlet.2019.05.015
pii:
doi:
Substances chimiques
Antibodies, Antinuclear
0
Immunoglobulin A
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
53-59Informations de copyright
Copyright © 2019 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.