A Cellular-Resolution Atlas of the Larval Zebrafish Brain.
brain networks
cerebellum
connectomics
digital atlas
neuroanatomy
single-cell tracing
tectum
tissue clearing
Journal
Neuron
ISSN: 1097-4199
Titre abrégé: Neuron
Pays: United States
ID NLM: 8809320
Informations de publication
Date de publication:
03 07 2019
03 07 2019
Historique:
received:
18
09
2018
revised:
10
04
2019
accepted:
23
04
2019
pubmed:
31
5
2019
medline:
23
10
2019
entrez:
1
6
2019
Statut:
ppublish
Résumé
Understanding brain-wide neuronal dynamics requires a detailed map of the underlying circuit architecture. We built an interactive cellular-resolution atlas of the zebrafish brain at 6 days post-fertilization (dpf) based on the reconstructions of over 2,000 individually GFP-labeled neurons. We clustered our dataset in "morphotypes," establishing a unique database of quantitatively described neuronal morphologies together with their spatial coordinates in vivo. Over 100 transgene expression patterns were imaged separately and co-registered with the single-neuron atlas. By annotating 72 non-overlapping brain regions, we generated from our dataset an inter-areal wiring diagram of the larval brain, which serves as ground truth for synapse-scale, electron microscopic reconstructions. Interrogating our atlas by "virtual tract tracing" has already revealed previously unknown wiring principles in the tectum and the cerebellum. In conclusion, we present here an evolving computational resource and visualization tool, which will be essential to map function to structure in a vertebrate brain. VIDEO ABSTRACT.
Identifiants
pubmed: 31147152
pii: S0896-6273(19)30391-5
doi: 10.1016/j.neuron.2019.04.034
pii:
doi:
Substances chimiques
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
21-38.e5Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.