Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models.
Adhesion
Blister
Cell culture
Epidermolysis bullosa
Fibroblast
Keratinocyte
Organotypic coculture
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
1
6
2019
pubmed:
31
5
2019
medline:
27
2
2020
Statut:
ppublish
Résumé
Isolation and culture of keratinocytes from patients with different types of epidermolysis bullosa are sometimes challenging, because of the inherent adhesion defects of these cells. We routinely employ a well-established protocol for in vitro culture of these cells from small skin samples remaining after diagnostic procedures are performed. Keratinocytes and fibroblast can be used for downstream expression and functional studies or for construction of in vitro organotypic cocultures. These cells maintain main common characteristics of spreading, adhesion, migration, and survival, which depend on the underlying molecular defect.
Identifiants
pubmed: 31148087
doi: 10.1007/978-1-4939-9473-1_14
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
181-190Références
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