In vitro proinflammatory gene expression changes in human whole blood after contact with plasma-treated implant surfaces.
Atmospheric-pressure plasma
Dental implants
Gene expression analysis
Human whole blood
Low-pressure plasma
Proinflammatory cytokines
Journal
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery
ISSN: 1878-4119
Titre abrégé: J Craniomaxillofac Surg
Pays: Scotland
ID NLM: 8704309
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
23
12
2018
revised:
28
04
2019
accepted:
06
05
2019
pubmed:
4
6
2019
medline:
18
12
2019
entrez:
2
6
2019
Statut:
ppublish
Résumé
The aim of this in vitro study was to identify changes in gene expression of proinflammatory cytokines in human whole blood after contact with titanium implant surfaces after plasma treatment. Grade 4 titanium dental implants were conditioned with low-pressure plasma (LPP) and atmospheric-pressure plasma (APP) and submerged in human whole blood in vitro. Unconditioned implants and blood samples without implants served as control and negative control groups, respectively. Sampling was performed at 1, 8, and 24 h. Changes in mRNA expression levels of interleukin 1-beta (IL1-β) and tumor necrosis factor-alpha (TNF-α) were assessed using RT-qPCR. In the control group, significant increases in IL1-β and TNF-α expression were observed. Significant decreases in the expression of IL1-β and TNF-α were identified in blood with implants after plasma treatment. Differences in gene expression of proinflammatory cytokines after incubation of plasma-conditioned titanium implants can be assessed using human whole blood. The results of the present study indicate that plasma treatment (APP and LPP) of titanium dental implants leads to downregulation of proinflammatory cytokine gene expression, which might be beneficial in early osseointegration.
Sections du résumé
BACKGROUND
BACKGROUND
The aim of this in vitro study was to identify changes in gene expression of proinflammatory cytokines in human whole blood after contact with titanium implant surfaces after plasma treatment.
MATERIALS AND METHODS
METHODS
Grade 4 titanium dental implants were conditioned with low-pressure plasma (LPP) and atmospheric-pressure plasma (APP) and submerged in human whole blood in vitro. Unconditioned implants and blood samples without implants served as control and negative control groups, respectively. Sampling was performed at 1, 8, and 24 h. Changes in mRNA expression levels of interleukin 1-beta (IL1-β) and tumor necrosis factor-alpha (TNF-α) were assessed using RT-qPCR.
RESULTS
RESULTS
In the control group, significant increases in IL1-β and TNF-α expression were observed. Significant decreases in the expression of IL1-β and TNF-α were identified in blood with implants after plasma treatment.
CONCLUSION
CONCLUSIONS
Differences in gene expression of proinflammatory cytokines after incubation of plasma-conditioned titanium implants can be assessed using human whole blood. The results of the present study indicate that plasma treatment (APP and LPP) of titanium dental implants leads to downregulation of proinflammatory cytokine gene expression, which might be beneficial in early osseointegration.
Identifiants
pubmed: 31151775
pii: S1010-5182(18)31156-9
doi: 10.1016/j.jcms.2019.05.004
pii:
doi:
Substances chimiques
Cytokines
0
Dental Implants
0
Titanium
D1JT611TNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1255-1261Informations de copyright
Copyright © 2019 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.