A point-of-care neutrophil elastase activity assay identifies bronchiectasis severity, airway infection and risk of exacerbation.


Journal

The European respiratory journal
ISSN: 1399-3003
Titre abrégé: Eur Respir J
Pays: England
ID NLM: 8803460

Informations de publication

Date de publication:
06 2019
Historique:
received: 12 02 2019
accepted: 14 03 2019
pubmed: 4 6 2019
medline: 30 10 2020
entrez: 2 6 2019
Statut: epublish

Résumé

Neutrophil elastase activity in sputum can identify patients at high risk of airway infection and exacerbations in bronchiectasis. Application of this biomarker in clinical practice is limited, because no point-of-care test is available. We tested whether a novel semi-quantitative lateral flow device (neutrophil elastase airway test stick - NEATstik®) can stratify bronchiectasis patients according to severity, airway infection and exacerbation risk. Sputum samples from 124 patients with stable bronchiectasis enrolled in the UK and Spain were tested using the NEATstik®, which scores neutrophil elastase concentration from 0 (<8 µg·mL Patients had a median age of 69 years and forced expiratory volume in 1 s (FEV A novel lateral flow device provides assessment of neutrophil elastase activity from sputum in minutes and identifies patients at increasing risk of airway infection and future exacerbations.

Identifiants

pubmed: 31151955
pii: 13993003.00303-2019
doi: 10.1183/13993003.00303-2019
pii:
doi:

Substances chimiques

Biomarkers 0
Leukocyte Elastase EC 3.4.21.37

Types de publication

Journal Article Multicenter Study Research Support, Non-U.S. Gov't Validation Study

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Chief Scientist Office
ID : SCAF/17/03
Pays : United Kingdom

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright ©ERS 2019.

Déclaration de conflit d'intérêts

Conflict of interest: A. Shoemark has nothing to disclose. Conflict of interest: E. Cant has nothing to disclose. Conflict of interest: L. Carreto has nothing to disclose. Conflict of interest: A. Smith has nothing to disclose. Conflict of interest: M. Oriano has nothing to disclose. Conflict of interest: H.R. Keir has nothing to disclose. Conflict of interest: L. Perea has nothing to disclose. Conflict of interest: E. Canto has nothing to disclose. Conflict of interest: L. Terranova has nothing to disclose. Conflict of interest: S. Vidal has nothing to disclose. Conflict of interest: K. Moffitt was employed by Proaxsis, during the conduct of the study. Conflict of interest: S. Aliberti has nothing to disclose. Conflict of interest: O Sibila has nothing to disclose. Conflict of interest: J.D. Chalmers reports grants and personal fees from GlaxoSmithKline, Boehringer Ingelheim, Pfizer, Bayer Healthcare, Grifols and Insmed, grants from AstraZeneca, personal fees from Napp and Aradigm Corporation, outside the submitted work.

Auteurs

Amelia Shoemark (A)

Scottish Centre for Respiratory Research, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK.

Erin Cant (E)

Scottish Centre for Respiratory Research, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK.

Luis Carreto (L)

Hospital Professor Fernando Fonseca (HFF), Lisbon, Portugal.

Alexandria Smith (A)

University of Cambridge, Cambridge, UK.

Martina Oriano (M)

Dept of Pathophysiology and Transplantation, University of Milan and Fondazione IRCCS Ca'Granda Ospedale Maggiore Policlinico, Respiratory Unit and Cystic Fibrosis Adult Center, Milan, Italy.

Holly R Keir (HR)

Scottish Centre for Respiratory Research, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK.

Lidia Perea (L)

Hospital de la Santa Creu I Sant Pau, Biomedical Research Institute (IIB-Sant Pau) Barcelona, Barcelona, Spain.

Elisabet Canto (E)

Hospital de la Santa Creu I Sant Pau, Biomedical Research Institute (IIB-Sant Pau) Barcelona, Barcelona, Spain.

Leonardo Terranova (L)

Dept of Pathophysiology and Transplantation, University of Milan and Fondazione IRCCS Ca'Granda Ospedale Maggiore Policlinico, Respiratory Unit and Cystic Fibrosis Adult Center, Milan, Italy.

Silvia Vidal (S)

Hospital de la Santa Creu I Sant Pau, Biomedical Research Institute (IIB-Sant Pau) Barcelona, Barcelona, Spain.

Kelly Moffitt (K)

Proaxsis Ltd, Belfast, UK.

Stefano Aliberti (S)

Dept of Pathophysiology and Transplantation, University of Milan and Fondazione IRCCS Ca'Granda Ospedale Maggiore Policlinico, Respiratory Unit and Cystic Fibrosis Adult Center, Milan, Italy.

Oriol Sibila (O)

Hospital de la Santa Creu I Sant Pau, Biomedical Research Institute (IIB-Sant Pau) Barcelona, Barcelona, Spain.

James D Chalmers (JD)

Scottish Centre for Respiratory Research, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK.

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Classifications MeSH