Biochemical study of sortase E2 from Streptomyces mobaraensis and determination of transglutaminase cross-linking sites.

Aminoacyltransferases / chemistry Bacterial Proteins / chemistry Cell Wall / metabolism Cysteine Endopeptidases / chemistry Glutamine / metabolism Oligopeptides / chemistry Protein Sorting Signals Solubility Streptomyces / enzymology Transglutaminases / genetics
Streptomyces mobaraensis chaplin glutamine donor proteins kinetics sortase transglutaminase

Journal

FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157

Informations de publication

Date de publication:
08 2019
Historique:
received: 03 04 2019
revised: 27 05 2019
accepted: 29 05 2019
pubmed: 4 6 2019
medline: 5 6 2020
entrez: 4 6 2019
Statut: ppublish

Résumé

Distinct streptomycetes such as Streptomyces mobaraensis produce the protein cross-linking enzyme transglutaminase. Bioinformatic analysis predicted the occurrence of seven sortases exerting transpeptidation reactions similarly to transglutaminase. Here, we report the production and characterization of sortase E2 (Sm-SrtE2) solubilized by removal of its membrane anchor domain. Sm-SrtE2 activity was measured using pentapeptides predicted to be cell wall sorting signals of putative sortase substrate proteins. Preferred linkage to Gly

Identifiants

DOI: 10.1002/1873-3468.13466 PMID: 31155711
pubmed: 31155711
doi: 10.1002/1873-3468.13466
doi:

Substances chimiques

Bacterial Proteins 0
Oligopeptides 0
Protein Sorting Signals 0
sortase E 0
Glutamine 0RH81L854J
Aminoacyltransferases EC 2.3.2.-
Transglutaminases EC 2.3.2.13
Cysteine Endopeptidases EC 3.4.22.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1944-1956

Subventions

Organisme : Graduate School of the University of Applied Sciences of Darmstadt
Pays : International
Organisme : German Federal Ministry of Education and Research
ID : 031A613C
Pays : International

Informations de copyright

© 2019 Federation of European Biochemical Societies.

Auteurs

Anita Anderl (A)

Department of Chemical Engineering and Biotechnology, University of Applied Sciences of Darmstadt, Germany.
Department of Chemistry, Technische Universität Darmstadt, Germany.

Cathrin Ferlemann (C)

Department of Chemical Engineering and Biotechnology, University of Applied Sciences of Darmstadt, Germany.

Marius Muth (M)

Department of Chemical Engineering and Biotechnology, University of Applied Sciences of Darmstadt, Germany.
Bioengineering and Biosystems, Institute of Functional Interfaces, Karlsruhe Institute of Technology, Germany.

Antonina Henkel-Gupalo (A)

Department of Chemical Engineering and Biotechnology, University of Applied Sciences of Darmstadt, Germany.

Aileen Ebenig (A)

Department of Chemistry, Technische Universität Darmstadt, Germany.

Gerald Brenner-Weiß (G)

Bioengineering and Biosystems, Institute of Functional Interfaces, Karlsruhe Institute of Technology, Germany.

Harald Kolmar (H)

Department of Chemistry, Technische Universität Darmstadt, Germany.

Hans-Lothar Fuchsbauer (HL)

Department of Chemical Engineering and Biotechnology, University of Applied Sciences of Darmstadt, Germany.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Aminoacyltransferases › Biochemical study of sortase E2 from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Biochemical study of sortase E2 from...
questionsmedicales.fr › Cellules › Structures cellulaires › Paroi cellulaire › Biochemical study of sortase E2 from...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Endopeptidases › Cysteine endopeptidases › Biochemical study of sortase E2 from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés neutres › Glutamine › Biochemical study of sortase E2 from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Oligopeptides › Biochemical study of sortase E2 from...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Signaux de triage des protéines › Biochemical study of sortase E2 from...
questionsmedicales.fr › Phénomènes chimiques › Solubilité › Biochemical study of sortase E2 from...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Streptomycetaceae › Streptomyces › Biochemical study of sortase E2 from...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Aminoacyltransferases › Transglutaminases › Biochemical study of sortase E2 from...