The transcription elongation factor TCEA3 promotes the activity of the myogenic regulatory factors.
Animals
Cell Differentiation
/ genetics
Cell Line
Cell Nucleus
/ metabolism
Cell Proliferation
/ genetics
Down-Regulation
/ genetics
Humans
Mice
Muscles
/ metabolism
Myogenic Regulatory Factors
/ metabolism
Organ Specificity
/ genetics
Promoter Regions, Genetic
/ genetics
Protein Binding
Protein Transport
RNA Polymerase II
/ metabolism
RNA, Messenger
/ genetics
Transcriptional Elongation Factors
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
25
02
2019
accepted:
17
05
2019
entrez:
4
6
2019
pubmed:
4
6
2019
medline:
12
2
2020
Statut:
epublish
Résumé
The transcription elongation factor TFIIS is encoded by a three member gene family in vertebrates. Here we show that one member of this family, TCEA3, is upregulated during skeletal muscle differentiation and acts to promote gene activation by the myogenic regulatory family of transcription factors, which includes MyoD and myogenin. We show that myogenin is a direct regulator of Tcea3. Myogenin binds to the Tcea3 promoter and is required to recruit RNA polymerase II. TCEA3 can bind to both myogenin and MyoD and is co-recruited with the MRFs to promoters dependent on the MRFs. Depletion of myogenin inhibits the recruitment of TCEA3, suggesting that the interaction of TCEA3 with the MRFs serves to aid in recruitment to target promoters. Like TFIIS, we show that TCEA3 interacts with RNA polymerase II. TCEA3 travels with the elongating RNA polymerase II in the coding region of genes and depletions of TCEA3 inhibit the recruitment of RNA polymerase II to promoters. In proliferating cells, TCEA3 expressed at low levels and is present in both the nucleus and cytoplasm. However, upon differentiation, TCEA3 is upregulated and transported exclusively to the nucleus. Thus, our data show that TCEA3 is a required co-factor for MRF driven gene expression during myogenesis.
Identifiants
pubmed: 31158246
doi: 10.1371/journal.pone.0217680
pii: PONE-D-19-05590
pmc: PMC6546274
doi:
Substances chimiques
Myogenic Regulatory Factors
0
RNA, Messenger
0
Transcriptional Elongation Factors
0
transcription factor S-II
0
RNA Polymerase II
EC 2.7.7.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0217680Subventions
Organisme : NIAMS NIH HHS
ID : R15 AR068622
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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