Site-Specific Labeling of Proteins Using the Formylglycine-Generating Enzyme (FGE).
Amino Acid Sequence
Amino Acids
/ chemistry
Consensus Sequence
Humans
Immunoconjugates
/ chemistry
Immunoglobulin Heavy Chains
/ chemistry
Oxidoreductases Acting on Sulfur Group Donors
/ chemistry
Protein Processing, Post-Translational
Proteins
/ chemistry
Staining and Labeling
Structure-Activity Relationship
Bioconjugation
FGE
Formylglycine
Formylglycine generating enzyme
Noncanonical amino acid
Posttranslational modification
Protein labeling
SUMF1
Unnatural amino acid
fGly
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
5
6
2019
pubmed:
5
6
2019
medline:
19
3
2020
Statut:
ppublish
Résumé
Use of the formylglycine generating enzyme (FGE)-a copper-dependent posttranslational protein modifier-represents a particularly elegant method taken directly from nature of introducing a unique amino acid into the larger context of a protein. Formylglycine (fGly) is a crucial component of the active site of sulfatases, where it directly participates in the breakdown of sulfate ester substrates. In the context of bioconjugation this aldehyde containing amino acid can be an invaluable reactive handle for the chemical conjugation of molecules. Here we describe a detailed method for generating formylglycine-containing proteins in a mammalian system developed specifically for the production of antibody-drug conjugates (ADCs) but applicable to a wide range of proteins.
Identifiants
pubmed: 31161504
doi: 10.1007/978-1-4939-9546-2_5
doi:
Substances chimiques
Amino Acids
0
Immunoconjugates
0
Immunoglobulin Heavy Chains
0
Proteins
0
Oxidoreductases Acting on Sulfur Group Donors
EC 1.8.-
SUMF1 protein, human
EC 3.1.6.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
63-81Références
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