Galactofuranosidase from JHA 19 Streptomyces sp.: subcloning and biochemical characterization.
Glycoside hydrolase
Subcloning
Thio-β-D-galactofuranoside inhibition
β-D-galactofuranosidase
Journal
Carbohydrate research
ISSN: 1873-426X
Titre abrégé: Carbohydr Res
Pays: Netherlands
ID NLM: 0043535
Informations de publication
Date de publication:
01 Jul 2019
01 Jul 2019
Historique:
received:
23
04
2019
revised:
16
05
2019
accepted:
22
05
2019
pubmed:
8
6
2019
medline:
22
11
2019
entrez:
8
6
2019
Statut:
ppublish
Résumé
Despite the crucial role of the rare galactofuranose (Galf) in many pathogenic micro-organisms and our increased knowledge of its metabolism, there is still a lack of recombinant and efficient galactofuranoside hydrolase available for chemo-enzymatic synthetic purposes of specific galactofuranosyl-conjugates. Subcloning of the Galf-ase from JHA 19 Streptomyces sp. and its further overexpression lead us to the production of this enzyme with a yield of 0.5 mg/L of culture. It exhibits substrate specificity exclusively towards pNP β-d-Galf, giving a K
Identifiants
pubmed: 31174175
pii: S0008-6215(19)30267-8
doi: 10.1016/j.carres.2019.05.011
pii:
doi:
Substances chimiques
Glycoside Hydrolases
EC 3.2.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
35-41Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.