Multiplex polymerase chain reaction for detection of bacteremia during dental extraction.
bacteremia
dental extraction
microbial culture
multiplex polymerase chain reaction
Journal
Journal of investigative and clinical dentistry
ISSN: 2041-1626
Titre abrégé: J Investig Clin Dent
Pays: Australia
ID NLM: 101524471
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
27
02
2019
revised:
20
04
2019
accepted:
27
04
2019
pubmed:
9
6
2019
medline:
27
11
2019
entrez:
9
6
2019
Statut:
ppublish
Résumé
Incidence of transient bacteremia following dental extractions ranges 30%-70% among adults and 58%-100% in children. This study aims to assess the multiplex polymerase chain reaction (PCR) technique in detection of transient bacteremia during dental extraction. Twenty volunteers for dental extraction entered the study. Blood samples were taken at baseline and 30 seconds and 15 minutes after extraction. Five types of bacteria were selected, namely Streptococcus mutans, S. salivarius, S. sanguinis, Enterococcus faecalis and Lactobacillus. Blood samples were evaluated by microbial culture and multiplex PCR. Blood culture showed rates of 0%, 80% and 25% for bacteremia before, during and after dental extraction, respectively. A significant difference was observed between baseline and during extraction. Using PCR, bacteremia was deemed 20%, 100% and 30% before, during and after extraction, respectively, and a significant difference was witnessed between the baseline and during extraction. The highest incidence was at the 30-second mark after extraction, and the most prevalent type of bacteria was S. mutans. Multiplex PCR can be used to determine bacterial diversity with high accuracy during occurrence of bacteremia. Therefore, in cases where positive blood cultures only indicate the presence of one species, we recommend the use of this method to detect more diverse bacteria types.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12425Subventions
Organisme : Shiraz University of Medical Sciences
ID : 96-01-03-14816
Informations de copyright
© 2019 John Wiley & Sons Australia, Ltd.
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