Emergence of plasmid stability under non-selective conditions maintains antibiotic resistance.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 06 2019
13 06 2019
Historique:
received:
12
01
2019
accepted:
21
05
2019
entrez:
15
6
2019
pubmed:
15
6
2019
medline:
10
7
2019
Statut:
epublish
Résumé
Plasmid acquisition is an important mechanism of rapid adaptation and niche expansion in prokaryotes. Positive selection for plasmid-coded functions is a major driver of plasmid evolution, while plasmids that do not confer a selective advantage are considered costly and expected to go extinct. Yet, plasmids are ubiquitous in nature, and their persistence remains an evolutionary paradox. Here, we demonstrate that non-mobile plasmids persist over evolutionary timescales without selection for the plasmid function. Evolving a minimal plasmid encoding for antibiotics resistance in Escherichia coli, we discover that plasmid stability emerges in the absence of antibiotics and that plasmid loss is determined by transcription-replication conflicts. We further find that environmental conditions modulate these conflicts and plasmid persistence. Silencing the transcription of the resistance gene results in stable plasmids that become fixed in the population. Evolution of plasmid stability under non-selective conditions provides an evolutionary explanation for the ubiquity of plasmids in nature.
Identifiants
pubmed: 31197163
doi: 10.1038/s41467-019-10600-7
pii: 10.1038/s41467-019-10600-7
pmc: PMC6565834
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2595Subventions
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : 640384
Pays : International
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : DA1202/2-1
Pays : International
Références
Nucleic Acids Res. 2016 Jul 8;44(W1):W242-5
pubmed: 27095192
Mol Microbiol. 2008 Jan;67(2):241-53
pubmed: 18047567
mBio. 2014 Oct 07;5(5):e01918-14
pubmed: 25293762
J Mol Biol. 1983 Jun 5;166(4):557-80
pubmed: 6345791
Trends Ecol Evol. 2014 Jan;29(1):33-41
pubmed: 24148292
Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):8260-5
pubmed: 27385827
Trends Microbiol. 2012 Jun;20(6):262-7
pubmed: 22564249
Mol Biol Evol. 2016 Nov;33(11):2860-2873
pubmed: 27501945
Nat Commun. 2014 Oct 10;5:5208
pubmed: 25302567
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
ISME J. 2017 Apr;11(4):1043-1052
pubmed: 27922600
Microb Cell Fact. 2008 Mar 07;7:6
pubmed: 18328094
Nat Ecol Evol. 2017 Sep;1(9):1354-1363
pubmed: 29046540
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
BMC Microbiol. 2010 Feb 19;10:56
pubmed: 20170524
Genetics. 2000 Aug;155(4):1505-19
pubmed: 10924453
Microbiology (Reading). 2007 Feb;153(Pt 2):452-463
pubmed: 17259616
Genome Res. 2010 Sep;20(9):1297-303
pubmed: 20644199
Nat Rev Genet. 2013 Dec;14(12):827-39
pubmed: 24166031
Nature. 1988 Sep 22;335(6188):351-2
pubmed: 3047585
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Microbiol Spectr. 2014 Oct;2(5):
pubmed: 26104344
Appl Environ Microbiol. 2012 Sep;78(17):6001-8
pubmed: 22706059
Cell. 1989 Jan 13;56(1):111-8
pubmed: 2535966
J Bacteriol. 2007 Mar;189(5):1946-53
pubmed: 17158664
Cell. 1989 Feb 24;56(4):521-3
pubmed: 2645054
Nat Ecol Evol. 2017 Jun 19;1:193
pubmed: 29388565
Curr Biol. 2015 Aug 3;25(15):2034-9
pubmed: 26190075
Nat Commun. 2015 Apr 21;6:6845
pubmed: 25897488
Nat Rev Genet. 2012 Feb 14;13(3):204-14
pubmed: 22330764
ISME J. 2018 Dec;12(12):3014-3024
pubmed: 30097663
BMC Mol Biol. 2011 Apr 23;12:18
pubmed: 21513543
Nucleic Acids Res. 2018 Jan 25;46(2):504-519
pubmed: 29202195
Microbiol Spectr. 2017 Sep;5(5):
pubmed: 28944751
EMBO J. 1982;1(11):1399-404
pubmed: 6327267
Cell. 1976 Dec;9(4 PT 2):707-16
pubmed: 797459
Sci Rep. 2017 Jul 7;7(1):4853
pubmed: 28687759
Appl Environ Microbiol. 2015 Sep 1;81(17):5976-86
pubmed: 26116677
J Antimicrob Chemother. 2018 May 1;73(5):1121-1137
pubmed: 29370371
Mol Biol Evol. 2019 Mar 1;36(3):472-486
pubmed: 30517696
Genetics. 1996 May;143(1):15-26
pubmed: 8722758
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4046-50
pubmed: 6377307
Mol Biol Evol. 2016 Apr;33(4):885-97
pubmed: 26668183
Clin Microbiol Rev. 2018 Aug 1;31(4):
pubmed: 30068738
Mol Microbiol. 1992 Jul;6(13):1785-99
pubmed: 1321324
Genetics. 2008 Apr;178(4):2179-90
pubmed: 18430943
Nat Rev Microbiol. 2012 Jun 06;10(7):449-58
pubmed: 22669220
FEMS Microbiol Ecol. 2018 Jan 1;94(1):
pubmed: 29228229
Mol Biol Evol. 2015 Jan;32(1):268-74
pubmed: 25371430
J Bacteriol. 1952 Mar;63(3):399-406
pubmed: 14927572
Mol Microbiol. 1993 Jun;8(6):1031-8
pubmed: 8361350
Microbiol Mol Biol Rev. 2010 Sep;74(3):434-52
pubmed: 20805406
Nucleic Acids Res. 2012 Dec;40(22):11189-201
pubmed: 23066108
Proc Biol Sci. 2018 Jan 10;285(1870):
pubmed: 29321301
FEMS Microbiol Ecol. 2007 Mar;59(3):738-48
pubmed: 17059480
Cell. 1984 Apr;36(4):1097-103
pubmed: 6323019
Gene. 1995 Dec 1;166(1):175-6
pubmed: 8529885
Genetics. 2007 Jun;176(2):957-68
pubmed: 17151258
Biophys J. 2016 Aug 9;111(3):467-479
pubmed: 27508432
Front Genet. 2017 Dec 15;8:211
pubmed: 29326751
Trends Microbiol. 2017 Jul;25(7):515-521
pubmed: 28216294
Curr Biol. 2015 Aug 31;25(17):R764-7
pubmed: 26325139
Plasmid. 2013 Nov;70(3):353-61
pubmed: 24042048
Biophys Rev. 2016 Sep;8(3):209-220
pubmed: 28510224
ISME J. 2018 Aug;12(8):1994-2010
pubmed: 29795276
Mob Genet Elements. 2016 May 04;6(3):e1179074
pubmed: 27510852
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
ISME J. 2010 Dec;4(12):1568-80
pubmed: 20520653
BMC Microbiol. 2012 Apr 04;12:53
pubmed: 22475035
Plasmid. 1996 Nov;36(3):161-7
pubmed: 9007011
Nature. 2016 Jul 7;535(7610):178-81
pubmed: 27362223
Gene. 1982 Oct;19(3):327-36
pubmed: 6295884
Genetics. 2007 Sep;177(1):399-405
pubmed: 17890367
PLoS One. 2014 Feb 04;9(2):e87924
pubmed: 24503942
J Bacteriol. 2015 Jul;197(14):2374-82
pubmed: 25939832
Trends Microbiol. 2018 Dec;26(12):978-985
pubmed: 30049587
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Nat Struct Mol Biol. 2013 Apr;20(4):412-8
pubmed: 23552296
Biophys J. 2009 Jun 17;96(12):4951-5
pubmed: 19527654
Proc Natl Acad Sci U S A. 2006 May 9;103(19):7310-4
pubmed: 16648255
J Bacteriol. 1995 Jul;177(14):4121-30
pubmed: 7608087
Nucleic Acids Res. 2014 Jan;42(2):1042-51
pubmed: 24137005