Complementary Approaches to Imaging Subcellular Lipid Architectures in Live Bacteria Using Phosphorescent Iridium Complexes and Raman Spectroscopy.

Bacillus cereus / metabolism Coordination Complexes / chemistry Iridium / chemistry Lipids / chemistry Luminescent Agents / chemistry Microscopy, Confocal / methods Spectrum Analysis, Raman
Raman spectroscopy bacterial imaging imaging agents iridium luminescence

Journal

Chemistry (Weinheim an der Bergstrasse, Germany)
ISSN: 1521-3765
Titre abrégé: Chemistry
Pays: Germany
ID NLM: 9513783

Informations de publication

Date de publication:
09 Aug 2019
Historique:
received: 03 05 2019
revised: 11 06 2019
pubmed: 15 6 2019
medline: 4 9 2019
entrez: 15 6 2019
Statut: ppublish

Résumé

A family of three neutral iridium(III) tetrazolato complexes are investigated as bacterial imaging agents. The complexes offer a facile tuning of the emission colour from green (520 nm) to red (600 nm) in aqueous media, while keeping the excitation wavelength unchanged. The three complexes do not inhibit the bacterial growth of Bacillus Cereus, used as a model in this study, and exhibit extremely fast cellular uptake. After a minute incubation time, the nontoxic complexes show subcellular localisation in spherical structures identified as lipid vacuoles. Confocal Raman imaging has been exploited for the first time on live bacteria, to provide direct and label-free mapping of the lipid-enriched organelles within B. cereus, complementing the use of luminescent probes. Examination of the Raman spectra not only confirmed the presence of lipophilic inclusions in B. cereus but offered additional information about their chemical composition, suggesting that the lipid vacuoles may contain polyhydroxybutyrate (PHB).

Identifiants

DOI: 10.1002/chem.201902023 PMID: 31197892
pubmed: 31197892
doi: 10.1002/chem.201902023
doi:

Substances chimiques

Coordination Complexes 0
Lipids 0
Luminescent Agents 0
Iridium 44448S9773

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

10566-10570

Informations de copyright

© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Auteurs

Anna Maria Ranieri (AM)

Curtin Institute for Functional Molecules and Interfaces, and School of Molecular and Life Sciences, Curtin University, Bentley, 6102, WA, Australia.

Chiara Caporale (C)

Curtin Institute for Functional Molecules and Interfaces, and School of Molecular and Life Sciences, Curtin University, Bentley, 6102, WA, Australia.

Valentina Fiorini (V)

Department of Industrial Chemistry "Toso Montanari", University of Bologna, viale del Risorgimento4, 40136, Bologna, Italy.

Alysia Hubbard (A)

Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, 6009, WA, Australia.

Paul Rigby (P)

Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, 6009, WA, Australia.

Stefano Stagni (S)

Department of Industrial Chemistry "Toso Montanari", University of Bologna, viale del Risorgimento4, 40136, Bologna, Italy.

Elizabeth Watkin (E)

School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Kent Street, Bentley, 6102, Australia.

Mark I Ogden (MI)

Curtin Institute for Functional Molecules and Interfaces, and School of Molecular and Life Sciences, Curtin University, Bentley, 6102, WA, Australia.
ORCID: 0000-0001-5317-1637

Mark J Hackett (MJ)

Curtin Institute for Functional Molecules and Interfaces, and School of Molecular and Life Sciences, Curtin University, Bentley, 6102, WA, Australia.

Massimiliano Massi (M)

Curtin Institute for Functional Molecules and Interfaces, and School of Molecular and Life Sciences, Curtin University, Bentley, 6102, WA, Australia.
ORCID: 0000-0001-6949-4019

Articles similaires

Shining light on the Mary Rose: Identifying chemical differences in human aging and handedness in the clavicles of sailors using Raman spectroscopy.

Sheona Isobel Shankland, Alexzandra Hildred, Adam Michael Taylor et al.
1.00
Humans Spectrum Analysis, Raman Clavicle Male Adult

Synthesis and characterization of new organometallic lanthanides metal complexes for photodynamic therapy.

H A Fetouh, E H El-Mossalamy, J M El Desouky et al.
1.00
Photochemotherapy Lanthanoid Series Elements Humans Coordination Complexes Schiff Bases

Reducing the mitochondrial oxidative burden alleviates lipid-induced muscle insulin resistance in humans.

Matteo Fiorenza, Johan Onslev, Carlos Henríquez-Olguín et al.
1.00
Humans Insulin Resistance Muscle, Skeletal Oxidative Stress Oxidation-Reduction

Gender-specific association between a lipid composite index and asthma among US adults: insights from a population-based study.

Bufan Ying, Xiaoxin Liu, Chengming Yang et al.
1.00
Humans Asthma Male Female Middle Aged

Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus cereus › Complementary Approaches to Imaging...
questionsmedicales.fr › Composés chimiques organiques › Complexes de coordination › Complementary Approaches to Imaging...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Iridium › Complementary Approaches to Imaging...
questionsmedicales.fr › Lipides › Complementary Approaches to Imaging...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Complementary Approaches to Imaging...
questionsmedicales.fr › Techniques d'investigation › Microscopie confocale › Complementary Approaches to Imaging...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Analyse spectrale Raman › Complementary Approaches to Imaging...