Spatiotemporally Super-Resolved Volumetric Traction Force Microscopy.
3D SIM
Traction force microscopy
actin cytoskeleton
biomechanics
super-resolution
Journal
Nano letters
ISSN: 1530-6992
Titre abrégé: Nano Lett
Pays: United States
ID NLM: 101088070
Informations de publication
Date de publication:
10 07 2019
10 07 2019
Historique:
pubmed:
15
6
2019
medline:
9
4
2020
entrez:
15
6
2019
Statut:
ppublish
Résumé
Quantification of mechanical forces is a major challenge across biomedical sciences. Yet such measurements are essential to understanding the role of biomechanics in cell regulation and function. Traction force microscopy remains the most broadly applied force probing technology but typically restricts itself to single-plane two-dimensional quantifications with limited spatiotemporal resolution. Here, we introduce an enhanced force measurement technique combining 3D super-resolution fluorescence structural illumination microscopy and traction force microscopy (3D-SIM-TFM) offering increased spatiotemporal resolution, opening-up unprecedented insights into physiological three-dimensional force production in living cells.
Identifiants
pubmed: 31199151
doi: 10.1021/acs.nanolett.9b01196
pmc: PMC6628183
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4427-4434Références
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