Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach.
Animals
Biomarkers
Hepatocyte Growth Factor
/ genetics
Humans
Immunohistochemistry
Mice
Mice, Transgenic
Myocytes, Smooth Muscle
/ cytology
Organogenesis
/ genetics
Phosphorylation
Protein Transport
Proto-Oncogene Proteins c-met
/ metabolism
Regeneration
Stem Cells
/ cytology
Wound Healing
/ genetics
HGF
MET receptor
regeneration
smooth muscle cell
stem cell
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
17 Jun 2019
17 Jun 2019
Historique:
received:
21
05
2019
revised:
06
06
2019
accepted:
13
06
2019
entrez:
20
6
2019
pubmed:
20
6
2019
medline:
18
12
2019
Statut:
epublish
Résumé
Hepatocyte growth factor (HGF) is secreted as an inactive single-chain HGF (scHGF); however, only proteolytically processed two-chain HGF (tcHGF) can activate the MET receptor. We investigated the localization of tcHGF and activated/phosphorylated MET (pMET) using a tcHGF-specific antibody. In day 16.5 mouse embryos, total HGF (scHGF + tcHGF) was mainly localized in smooth muscle cells close to, but separate from, MET-positive epithelial cells in endodermal organs, including the stomach. In the adult stomach, total HGF was localized in smooth muscle cells, and tcHGF was mainly localized in the glandular base region. Immunostaining for pMET and Lgr5-driven green fluorescent protein (GFP) indicated that pMET localization overlapped with Lgr5
Identifiants
pubmed: 31212972
pii: ijms20122955
doi: 10.3390/ijms20122955
pmc: PMC6628191
pii:
doi:
Substances chimiques
Biomarkers
0
Hepatocyte Growth Factor
67256-21-7
Proto-Oncogene Proteins c-met
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Agency for Medical Research and Development
ID : 16cm0106201h0001
Organisme : Japan Agency for Medical Research and Development
ID : 16am0301001h0003
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