Reversible ADP-ribosylation of RNA.
ADP Ribose Transferases
/ genetics
ADP-Ribosylation
Adenosine Diphosphate
/ chemistry
Adenosine Diphosphate Ribose
Animals
Catalysis
Chromatin
/ chemistry
DNA Repair
DNA Repair Enzymes
/ metabolism
DNA, Single-Stranded
/ metabolism
Escherichia coli
/ metabolism
Humans
Hydrolases
/ metabolism
Mice
NAD
/ metabolism
Phosphorylation
Phosphotransferases (Alcohol Group Acceptor)
/ chemistry
Plasmids
/ metabolism
Poly(ADP-ribose) Polymerases
/ metabolism
Protein Processing, Post-Translational
Proto-Oncogene Proteins
/ metabolism
RNA
/ metabolism
Signal Transduction
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
20 06 2019
20 06 2019
Historique:
accepted:
23
04
2019
revised:
10
04
2019
received:
01
12
2018
entrez:
20
6
2019
pubmed:
20
6
2019
medline:
4
12
2019
Statut:
ppublish
Résumé
ADP-ribosylation is a reversible chemical modification catalysed by ADP-ribosyltransferases such as PARPs that utilize nicotinamide adenine dinucleotide (NAD+) as a cofactor to transfer monomer or polymers of ADP-ribose nucleotide onto macromolecular targets such as proteins and DNA. ADP-ribosylation plays an important role in several biological processes such as DNA repair, transcription, chromatin remodelling, host-virus interactions, cellular stress response and many more. Using biochemical methods we identify RNA as a novel target of reversible mono-ADP-ribosylation. We demonstrate that the human PARPs - PARP10, PARP11 and PARP15 as well as a highly diverged PARP homologue TRPT1, ADP-ribosylate phosphorylated ends of RNA. We further reveal that ADP-ribosylation of RNA mediated by PARP10 and TRPT1 can be efficiently reversed by several cellular ADP-ribosylhydrolases (PARG, TARG1, MACROD1, MACROD2 and ARH3), as well as by MACROD-like hydrolases from VEEV and SARS viruses. Finally, we show that TRPT1 and MACROD homologues in bacteria possess activities equivalent to the human proteins. Our data suggest that RNA ADP-ribosylation may represent a widespread and physiologically relevant form of reversible ADP-ribosylation signalling.
Identifiants
pubmed: 31216043
pii: 5480135
doi: 10.1093/nar/gkz305
pmc: PMC6582358
doi:
Substances chimiques
Chromatin
0
DNA, Single-Stranded
0
MACROD2 protein, human
0
Proto-Oncogene Proteins
0
NAD
0U46U6E8UK
Adenosine Diphosphate Ribose
20762-30-5
Adenosine Diphosphate
61D2G4IYVH
RNA
63231-63-0
ADP Ribose Transferases
EC 2.4.2.-
PARP10 protein, human
EC 2.4.2.30
Poly(ADP-ribose) Polymerases
EC 2.4.2.30
Phosphotransferases (Alcohol Group Acceptor)
EC 2.7.1.-
Trpt1 protein, mouse
EC 2.7.1.-
Hydrolases
EC 3.-
DNA Repair Enzymes
EC 6.5.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5658-5669Subventions
Organisme : Wellcome Trust
ID : 101794
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 210634
Pays : United Kingdom
Organisme : NINDS NIH HHS
ID : R01 NS088629
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
J Virol. 2014 Feb;88(4):2116-30
pubmed: 24335297
Front Microbiol. 2018 Jan 23;9:20
pubmed: 29410655
J Virol. 2012 Aug;86(15):8147-60
pubmed: 22623789
Mol Biosyst. 2012 Jun;8(6):1661-77
pubmed: 22399070
Trends Microbiol. 2018 Jul;26(7):598-610
pubmed: 29268982
EMBO J. 2013 May 2;32(9):1225-37
pubmed: 23481255
Nat Commun. 2014 Jul 21;5:4426
pubmed: 25043379
J Biol Chem. 1997 May 16;272(20):13203-10
pubmed: 9148937
J Virol. 2016 Sep 12;90(19):8478-86
pubmed: 27440879
FEBS J. 2006 Oct;273(20):4579-93
pubmed: 16956368
Elife. 2018 Feb 26;7:
pubmed: 29480802
DNA Repair (Amst). 2014 Nov;23:4-16
pubmed: 24865146
Biochem J. 2015 Jun 1;468(2):293-301
pubmed: 25789582
Nat Struct Mol Biol. 2013 Apr;20(4):508-14
pubmed: 23474712
Nat Rev Mol Cell Biol. 2012 Jun 20;13(7):411-24
pubmed: 22713970
Cell Rep. 2016 Jan 26;14(3):621-631
pubmed: 26774478
J Biol Chem. 2001 Sep 21;276(38):35891-9
pubmed: 11454873
Nat Commun. 2013;4:1683
pubmed: 23575687
Nat Cell Biol. 2012 Nov;14(11):1223-30
pubmed: 23103912
Mol Cell. 2016 Dec 15;64(6):1109-1116
pubmed: 27939941
Mol Cell. 2015 Dec 3;60(5):755-768
pubmed: 26626480
Nat Struct Mol Biol. 2013 Apr;20(4):502-7
pubmed: 23474714
Nat Chem Biol. 2018 Feb 14;14(3):236-243
pubmed: 29443986
Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12414-9
pubmed: 11592983
FEBS J. 2017 Dec;284(23):4002-4016
pubmed: 29054115
Nat Chem Biol. 2016 Dec;12(12):998-1000
pubmed: 27723750
J Biol Chem. 1999 Jan 29;274(5):2637-44
pubmed: 9915792
Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1666-1671
pubmed: 28143925
Nucleic Acids Res. 2016 Nov 2;44(19):9279-9295
pubmed: 27471034
J Am Chem Soc. 2015 Mar 18;137(10):3558-64
pubmed: 25706250
FEBS J. 2016 Sep;283(18):3371-88
pubmed: 27406238
RNA. 2008 Feb;14(2):225-32
pubmed: 18094117
Eur J Med Chem. 2015 May 5;95:546-51
pubmed: 25847771
Mol Cell. 2008 Oct 10;32(1):57-69
pubmed: 18851833
Oncogene. 2005 Mar 17;24(12):1982-93
pubmed: 15674325
J Biol Chem. 2006 Jan 13;281(2):705-13
pubmed: 16278211
Sci Rep. 2018 Mar 8;8(1):4176
pubmed: 29520010
Nucleic Acids Res. 2018 Oct 12;46(18):9617-9624
pubmed: 30202863
J Biol Chem. 1991 Jun 25;266(18):11986-92
pubmed: 2050693
Crit Rev Biochem Mol Biol. 2018 Feb;53(1):64-82
pubmed: 29098880
Exp Cell Res. 2004 Jul 15;297(2):521-32
pubmed: 15212953
J Biol Chem. 2011 Apr 15;286(15):13261-71
pubmed: 21257746
RNA. 2005 Jan;11(1):107-13
pubmed: 15611301
Sci Rep. 2017 Feb 02;7:41746
pubmed: 28150709
J Bacteriol. 2000 Oct;182(19):5281-9
pubmed: 10986228
J Mol Biol. 2009 Jan 9;385(1):212-25
pubmed: 18983849
RNA. 2018 Sep;24(9):1144-1157
pubmed: 29884622
J Am Chem Soc. 2014 Apr 9;136(14):5201-4
pubmed: 24641686
Mol Cell. 2017 Mar 2;65(5):932-940.e6
pubmed: 28190768
Annu Rev Biochem. 2016 Jun 2;85:431-54
pubmed: 26844395
Elife. 2017 Jun 26;6:
pubmed: 28650317
FEBS J. 2017 Sep;284(18):2932-2946
pubmed: 28383827
Nature. 2011 Sep 04;477(7366):616-20
pubmed: 21892188
Cell Commun Signal. 2012 Sep 20;10(1):28
pubmed: 22992334
FEBS J. 2013 Mar;280(5):1330-43
pubmed: 23305266
Nucleic Acids Res. 2018 Mar 16;46(5):2417-2431
pubmed: 29361132
Mol Cell. 2016 May 5;62(3):432-442
pubmed: 27067600
J Biol Chem. 2014 May 9;289(19):13627-37
pubmed: 24695737
Trends Biochem Sci. 2010 Apr;35(4):208-19
pubmed: 20106667
mBio. 2016 Dec 13;7(6):
pubmed: 27965448