Small-molecule targeting of MUSASHI RNA-binding activity in acute myeloid leukemia.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
19 06 2019
Historique:
received: 19 11 2018
accepted: 16 05 2019
entrez: 21 6 2019
pubmed: 21 6 2019
medline: 10 7 2019
Statut: epublish

Résumé

The MUSASHI (MSI) family of RNA binding proteins (MSI1 and MSI2) contribute to a wide spectrum of cancers including acute myeloid leukemia. We find that the small molecule Ro 08-2750 (Ro) binds directly and selectively to MSI2 and competes for its RNA binding in biochemical assays. Ro treatment in mouse and human myeloid leukemia cells results in an increase in differentiation and apoptosis, inhibition of known MSI-targets, and a shared global gene expression signature similar to shRNA depletion of MSI2. Ro demonstrates in vivo inhibition of c-MYC and reduces disease burden in a murine AML leukemia model. Thus, we identify a small molecule that targets MSI's oncogenic activity. Our study provides a framework for targeting RNA binding proteins in cancer.

Identifiants

pubmed: 31217428
doi: 10.1038/s41467-019-10523-3
pii: 10.1038/s41467-019-10523-3
pmc: PMC6584500
doi:

Substances chimiques

2,3,4,10-tetrahydro-7,10-dimethyl-2,4-dioxobenzo(g)pteridine 0
Flavins 0
MYC protein, human 0
Msi2h protein, mouse 0
Proto-Oncogene Proteins c-myc 0
Pteridines 0
RNA, Small Interfering 0
RNA-Binding Proteins 0
RNA 63231-63-0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2691

Subventions

Organisme : U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI)
ID : P30-CA008748
Pays : International
Organisme : NIH HHS
ID : S10 OD016432
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA193842
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM121505
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK101989
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL135564
Pays : United States

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Auteurs

Gerard Minuesa (G)

Molecular Pharmacology Program, Experimental Therapeutics Center and Center for Stem Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA.

Steven K Albanese (SK)

Louis V. Gerstner Jr. Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.
Computational and Systems Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Wei Xie (W)

Structural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Yaniv Kazansky (Y)

Weill Cornell Medical College, Tri-Institutional MD-PhD Program, Rockefeller University and Sloan Kettering Institute, New York, NY, 10065, USA.

Daniel Worroll (D)

Department of Pharmacology, Weill Cornell Graduate School of Medical Sciences, New York, NY, 10065, USA.

Arthur Chow (A)

Molecular Pharmacology Program, Experimental Therapeutics Center and Center for Stem Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA.

Alexandra Schurer (A)

Molecular Pharmacology Program, Experimental Therapeutics Center and Center for Stem Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA.

Sun-Mi Park (SM)

Molecular Pharmacology Program, Experimental Therapeutics Center and Center for Stem Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA.

Christina Z Rotsides (CZ)

Chemical Biology Program, Sloan Kettering Institute and Tri-Institutional Research Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.

James Taggart (J)

Molecular Pharmacology Program, Experimental Therapeutics Center and Center for Stem Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA.

Andrea Rizzi (A)

Computational and Systems Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.
Tri-Institutional Training Program in Computational Biology and Medicine, Weill Cornell Medical College, New York, NY, 10065, USA.

Levi N Naden (LN)

Computational and Systems Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Timothy Chou (T)

Molecular Pharmacology Program, Experimental Therapeutics Center and Center for Stem Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA.

Saroj Gourkanti (S)

Molecular Pharmacology Program, Experimental Therapeutics Center and Center for Stem Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA.

Daniel Cappel (D)

Schrödinger GmbH, Q7, 23, 68161, Mannheim, Germany.

Maria C Passarelli (MC)

Computational and Systems Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.
Weill Cornell Medical College, Tri-Institutional MD-PhD Program, Rockefeller University and Sloan Kettering Institute, New York, NY, 10065, USA.

Lauren Fairchild (L)

Computational and Systems Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.
Tri-Institutional Training Program in Computational Biology and Medicine, Weill Cornell Medical College, New York, NY, 10065, USA.

Carolina Adura (C)

High-Throughput and Spectroscopy Resource Center, The Rockefeller University, New York, NY, 10065, USA.

J Fraser Glickman (JF)

High-Throughput and Spectroscopy Resource Center, The Rockefeller University, New York, NY, 10065, USA.

Jessica Schulman (J)

Hematologic Oncology Tissue Bank, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Christopher Famulare (C)

Center for Hematologic Malignancies, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Minal Patel (M)

Center for Hematologic Malignancies, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Joseph K Eibl (JK)

Northern Ontario School of Medicine, Sudbury, ON, P3E 2C6, Canada.

Gregory M Ross (GM)

Northern Ontario School of Medicine, Sudbury, ON, P3E 2C6, Canada.

Shibani Bhattacharya (S)

New York Structural Biology Center, NMR Group, New York, NY, 10027, USA.

Derek S Tan (DS)

Chemical Biology Program, Sloan Kettering Institute and Tri-Institutional Research Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.

Christina S Leslie (CS)

Computational and Systems Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Thijs Beuming (T)

Schrödinger, Inc., 120 West 45th Street, New York, NY, 10036, USA.

Dinshaw J Patel (DJ)

Structural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Yehuda Goldgur (Y)

Structural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

John D Chodera (JD)

Computational and Systems Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Michael G Kharas (MG)

Molecular Pharmacology Program, Experimental Therapeutics Center and Center for Stem Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA. kharasm@mskcc.org.

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