Endothelial lipase increases antioxidative capacity of high-density lipoprotein.
HDL
LDL
Mass spectrometry
Oxidation
Proteomics
Journal
Biochimica et biophysica acta. Molecular and cell biology of lipids
ISSN: 1879-2618
Titre abrégé: Biochim Biophys Acta Mol Cell Biol Lipids
Pays: Netherlands
ID NLM: 101731727
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
05
12
2018
revised:
31
05
2019
accepted:
14
06
2019
pubmed:
21
6
2019
medline:
7
3
2020
entrez:
21
6
2019
Statut:
ppublish
Résumé
Endothelial lipase (EL) is a strong determinant of structural and functional properties of high-density lipoprotein (HDL). We examined whether the antioxidative capacity of HDL is affected by EL. EL-modified HDL (EL-HDL) and control EV-HDL were generated by incubation of HDL with EL- overexpressing or control HepG2 cells. As determined by native gradient gel electrophoresis, electron microscopy, and small-angle X-ray scattering EL-HDL is smaller than EV-HDL. Mass spectrometry revealed an enrichment of EL-HDL with lipolytic products and depletion of phospholipids and triacylglycerol. Kinetics of conjugated diene formation and HPLC-based malondialdehyde quantification revealed that EL-HDL exhibited a significantly higher resistance to copper ion-induced oxidation and a significantly higher capacity to protect low-density lipoprotein (LDL) from copper ion-induced oxidation when compared to EV-HDL. Depletion of the lipolytic products from EL-HDL abolished the capacity of EL-HDL to protect LDL from copper ion-induced oxidation, which could be partially restored by lysophosphatidylcholine enrichment. Proteomics of HDL incubated with oxidized LDL revealed significantly higher levels of methionine 136 sulfoxide in EL-HDL compared to EV-HDL. Chloramine T (oxidizes methionines and modifies free thiols), diminished the difference between EL-HDL and EV-HDL regarding the capacity to protect LDL from oxidation. In absence of LDL small EV-HDL and EL-HDL exhibited higher resistance to copper ion-induced oxidation when compared to respective large particles. In conclusion, the augmented antioxidative capacity of EL-HDL is primarily determined by the enrichment of HDL with EL-generated lipolytic products and to a lesser extent by the decreased HDL particle size and the increased activity of chloramine T-sensitive mechanisms.
Identifiants
pubmed: 31220617
pii: S1388-1981(19)30114-3
doi: 10.1016/j.bbalip.2019.06.011
pmc: PMC6699986
mid: EMS83891
pii:
doi:
Substances chimiques
Lipoproteins, HDL
0
Copper
789U1901C5
LIPG protein, human
EC 3.1.1.3
Lipase
EC 3.1.1.3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1363-1374Subventions
Organisme : Austrian Science Fund FWF
ID : I 3792
Pays : Austria
Organisme : Austrian Science Fund FWF
ID : P 27166
Pays : Austria
Organisme : Austrian Science Fund FWF
ID : P 28854
Pays : Austria
Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier B.V. All rights reserved.
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