High-throughput screening for phosphatidylserine decarboxylase inhibitors using a distyrylbenzene-bis-aldehyde (DSB-3)-based fluorescence assay.
Candida albicans
/ drug effects
Carboxy-Lyases
/ antagonists & inhibitors
Cell Line, Tumor
Cell Survival
/ drug effects
Enzyme Inhibitors
/ analysis
Ethanolamine
/ pharmacology
Fluorescent Dyes
/ chemistry
High-Throughput Screening Assays
Humans
Inhibitory Concentration 50
Phosphatidylserines
/ metabolism
Plasmodium knowlesi
/ enzymology
Recombinant Proteins
/ biosynthesis
Styrenes
/ chemistry
Candida albicans
Plasmodium falciparum
Plasmodium knowlesi
antimicrobial agent
enzyme
high-throughput screening (HTS)
parasitic disease
phosphatidylethanolamine
phosphatidylserine
phosphatidylserine decarboxylase
phospholipids
plasmodium
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
09 08 2019
09 08 2019
Historique:
received:
27
01
2019
revised:
18
06
2019
pubmed:
23
6
2019
medline:
17
3
2020
entrez:
23
6
2019
Statut:
ppublish
Résumé
Phosphatidylserine decarboxylases (PSDs) catalyze the decarboxylation of phosphatidylserine to generate phosphatidylethanolamine, a critical step in phospholipid metabolism in both prokaryotes and eukaryotes. Most PSDs are membrane-bound, and classical radioisotope-based assays for determining their activity
Identifiants
pubmed: 31227523
pii: S0021-9258(20)30164-2
doi: 10.1074/jbc.RA119.007766
pmc: PMC6690695
pii:
doi:
Substances chimiques
Enzyme Inhibitors
0
Fluorescent Dyes
0
Phosphatidylserines
0
Recombinant Proteins
0
Styrenes
0
distyrylbenzene
0
Ethanolamine
5KV86114PT
Carboxy-Lyases
EC 4.1.1.-
phosphatidylserine decarboxylase
EC 4.1.1.65
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12146-12156Subventions
Organisme : NIAID NIH HHS
ID : R01 AI097218
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI123321
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001863
Pays : United States
Informations de copyright
© 2019 Hendricson et al.
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