The polysaccharide extracted from the biofilm of Burkholderia multivorans strain C1576 binds hydrophobic species and exhibits a compact 3D-structure.
AFM
Biofilm
Burkholderia multivorans
Conformation
Molecular interactions
Polysaccharide
Polysaccharide functions
SPR
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Sep 2019
01 Sep 2019
Historique:
received:
06
05
2019
revised:
13
06
2019
accepted:
19
06
2019
pubmed:
24
6
2019
medline:
17
1
2020
entrez:
24
6
2019
Statut:
ppublish
Résumé
Microorganisms often grow in communities called biofilms where cells are imbedded in a complex self-produced biopolymeric matrix composed mainly of polysaccharides, proteins, and DNA. This matrix, together with cell proximity, confers many advantages to these microbial communities, but also constitutes a serious concern when biofilms develop in human tissues or on implanted prostheses. Although polysaccharides are considered the main constituents of the matrices, their specific role needs to be clarified. We have investigated the chemical and morphological properties of the polysaccharide extracted from biofilms produced by the C1576 reference strain of the opportunistic pathogen Burkholderia multivorans, which causes lung infections in cystic fibrosis patients. The aim of the present study is the definition of possible interactions of the polysaccharide and the three-dimensional conformation of its chain within the biofilm matrix. Surface plasmon resonance experiments confirmed the ability of the polysaccharide to bind hydrophobic molecules, due to the presence of rhamnose dimers in its primary structure. In addition, atomic force microscopy studies evidenced an extremely compact three-dimensional structure of the polysaccharide which may form aggregates, suggesting a novel view of its structural role into the biofilm matrix.
Identifiants
pubmed: 31229548
pii: S0141-8130(19)33271-4
doi: 10.1016/j.ijbiomac.2019.06.140
pmc: PMC6711379
mid: NIHMS1043483
pii:
doi:
Substances chimiques
Alkanes
0
Polysaccharides, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
944-950Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM123283
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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