Catalyst-free Click PEGylation reveals substantial mitochondrial ATP synthase sub-unit alpha oxidation before and after fertilisation.

Adenosine Triphosphate / biosynthesis Amino Acid Sequence Animals Click Chemistry / methods Disulfides / chemistry Embryo, Nonmammalian Female Fertilization in Vitro Glutathione / metabolism Heterocyclic Compounds, 1-Ring / chemistry Male Mitochondria / chemistry Mitochondrial Proton-Translocating ATPases / chemistry Ovum / chemistry Oxidation-Reduction Phylogeny Polyethylene Glycols / chemistry Protein Processing, Post-Translational Protein Subunits / chemistry Sequence Alignment Sequence Homology, Amino Acid Sulfhydryl Compounds / chemistry Xenopus laevis / classification
ATP synthase Development Fertilisation Mitochondria Redox signalling

Journal

Redox biology
ISSN: 2213-2317
Titre abrégé: Redox Biol
Pays: Netherlands
ID NLM: 101605639

Informations de publication

Date de publication:
09 2019
Historique:
received: 20 05 2019
revised: 10 06 2019
accepted: 15 06 2019
pubmed: 25 6 2019
medline: 29 2 2020
entrez: 25 6 2019
Statut: ppublish

Résumé

Using non-reducing Western blotting to assess protein thiol redox state is challenging because most reduced and oxidised forms migrate at the same molecular weight and are, therefore, indistinguishable. While copper catalysed Click chemistry can be used to ligate a polyethylene glycol (PEG) moiety termed Click PEGylation to mass shift the reduced or oxidised form as desired, the potential for copper catalysed auto-oxidation is problematic. Here we define a catalyst-free trans-cyclooctene-methyltetrazine (TCO-Tz) inverse electron demand Diels Alder chemistry approach that affords rapid (k ~2000 M

Identifiants

DOI: 10.1016/j.redox.2019.101258 PMID: 31234016 PMC: PMC6597785
pubmed: 31234016
pii: S2213-2317(19)30563-4
doi: 10.1016/j.redox.2019.101258
pmc: PMC6597785
pii:
doi:

Substances chimiques

Disulfides 0
Heterocyclic Compounds, 1-Ring 0
Protein Subunits 0
Sulfhydryl Compounds 0
Polyethylene Glycols 3WJQ0SDW1A
Adenosine Triphosphate 8L70Q75FXE
Mitochondrial Proton-Translocating ATPases EC 3.6.3.-
Glutathione GAN16C9B8O

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101258

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 212942/Z/18/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R014841/1
Pays : United Kingdom

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

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Auteurs

James N Cobley (JN)

Free Radical Research Group, University of the Highlands and Islands, Centre for Health Sciences, Inverness, IV2 3JH, UK. Electronic address: james.cobley@uhi.ac.uk.

Anna Noble (A)

European Xenopus Resource Centre, University of Portsmouth, School of Biological Sciences, King Henry Building, Portsmouth, PO1 2DY, UK.

Eduardo Jimenez-Fernandez (E)

Free Radical Research Group, University of the Highlands and Islands, Centre for Health Sciences, Inverness, IV2 3JH, UK.

Manuel-Thomas Valdivia Moya (MT)

Free Radical Research Group, University of the Highlands and Islands, Centre for Health Sciences, Inverness, IV2 3JH, UK.

Matthew Guille (M)

European Xenopus Resource Centre, University of Portsmouth, School of Biological Sciences, King Henry Building, Portsmouth, PO1 2DY, UK.

Holger Husi (H)

Free Radical Research Group, University of the Highlands and Islands, Centre for Health Sciences, Inverness, IV2 3JH, UK.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › Adénosine triphosphate › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Eucaryotes › Animaux › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Chimie click › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Composés chimiques organiques › Composés du soufre › Sulfures › Disulfures › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Structures de l'embryon › Embryon non mammalien › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Techniques d'investigation › Techniques de reproduction › Techniques de reproduction assistée › Fécondation in vitro › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Oligopeptides › Glutathion › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines mitochondriales › Protéines de transport de la membrane mitochondriale › Mitochondrial Proton-Translocating ATPases › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Structures de l'embryon › Ovule › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Métabolisme › Métabolisme énergétique › Oxydoréduction › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Polyéthylène glycols › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Modification traductionnelle des protéines › Maturation post-traductionnelle des protéines › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Sous-unités de protéines › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Alignement de séquences › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Phénomènes génétiques › Similitude de séquences › Similitude de séquences d'acides aminés › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Composés chimiques organiques › Composés du soufre › Thiols › Catalyst-free Click PEGylation reveals...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Amphibiens › Anura › Pipidae › Xenopus › Xenopus laevis › Catalyst-free Click PEGylation reveals...