Purification and biochemical characterization of a novel thermostable and halotolerant subtilisin SAPN, a serine protease from Melghiribacillus thermohalophilus Nari2A

Animal Shells / chemistry Animals Bacillaceae / enzymology Bacterial Proteins / chemistry Chitin / chemistry Crustacea / chemistry Enzyme Stability Hydrolysis Salt Tolerance Subtilisin / chemistry Thermotolerance
Chitin Melghiribacillus thermohalophilus Metapenaeus monoceros Portunus segnis Subtilisin

Journal

Extremophiles : life under extreme conditions
ISSN: 1433-4909
Titre abrégé: Extremophiles
Pays: Germany
ID NLM: 9706854

Informations de publication

Date de publication:
Sep 2019
Historique:
received: 11 04 2019
accepted: 09 06 2019
pubmed: 27 6 2019
medline: 9 1 2020
entrez: 26 6 2019
Statut: ppublish

Résumé

The present study investigates the purification and biochemical characterization of a novel extracellular serine alkaline protease, subtilisin (called SAPN) from Melghiribacillus thermohalophilus Nari2A

Identifiants

DOI: 10.1007/s00792-019-01105-8 PMID: 31236718
pubmed: 31236718
doi: 10.1007/s00792-019-01105-8
pii: 10.1007/s00792-019-01105-8
doi:

Substances chimiques

Bacterial Proteins 0
Chitin 1398-61-4
Subtilisin EC 3.4.21.62

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

529-547

Subventions

Organisme : Ministère de l'Enseignement Supérieur, de la Recherche, de la Science et de la Technologie
ID : LMBEE-CBS/code: LR15CBS06 2015-2019

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Auteurs

Sondes Mechri (S)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.

Khelifa Bouacem (K)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.
Laboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), P.O. Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.

Fadoua Jabeur (F)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.

Sara Mohamed (S)

Laboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), P.O. Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.

Nariman Ammara Addou (NA)

Laboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), P.O. Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.

Ahlam Dab (A)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.

Aicha Bouraoui (A)

Laboratoire de Chimie Physique (LCP), CNRS UMR 8000, Faculté des Sciences, Université Paris-Sud, Université Paris-Saclay, 91405, Orsay Cedex, France.

Amel Bouanane-Darenfed (A)

Laboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), P.O. Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.

Samir Bejar (S)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.

Hocine Hacène (H)

Laboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), P.O. Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.

Laura Baciou (L)

Laboratoire de Chimie Physique (LCP), CNRS UMR 8000, Faculté des Sciences, Université Paris-Sud, Université Paris-Saclay, 91405, Orsay Cedex, France.

Florence Lederer (F)

Laboratoire de Chimie Physique (LCP), CNRS UMR 8000, Faculté des Sciences, Université Paris-Sud, Université Paris-Saclay, 91405, Orsay Cedex, France.

Bassem Jaouadi (B)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia. bassem.jaouadi@cbs.rnrt.tn.
Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia. bassem.jaouadi@cbs.rnrt.tn.

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Classifications MeSH

questionsmedicales.fr › Structures anatomiques de l'animal › Coquilles d'animaux › Purification and biochemical characterization...
questionsmedicales.fr › Eucaryotes › Animaux › Purification and biochemical characterization...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Purification and biochemical characterization...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Purification and biochemical characterization...
questionsmedicales.fr › Glucides › Polyosides › Chitine › Purification and biochemical characterization...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Crustacea › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Hydrolyse › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes biologiques › Adaptation biologique › Adaptation physiologique › Acclimatation › Tolérance au sel › Purification and biochemical characterization...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Protéases à sérine › Serine endopeptidases › Subtilisines › Subtilisine › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes biologiques › Adaptation biologique › Adaptation physiologique › Acclimatation › Thermotolérance › Purification and biochemical characterization...