T cells producing GM-CSF and IL-13 are enriched in the cerebrospinal fluid of relapsing MS patients.

Granulocyte-Macrophage Colony-Stimulating Factor Humans Interleukin-13 Multiple Sclerosis Multiple Sclerosis, Relapsing-Remitting Recurrence T-Lymphocytes
Multiple sclerosis T cells cerebrospinal fluid cytokines

Journal

Multiple sclerosis (Houndmills, Basingstoke, England)
ISSN: 1477-0970
Titre abrégé: Mult Scler
Pays: England
ID NLM: 9509185

Informations de publication

Date de publication:
09 2020
Historique:
pubmed: 27 6 2019
medline: 25 9 2021
entrez: 26 6 2019
Statut: ppublish

Résumé

Multiple sclerosis (MS) is a central nervous system (CNS) autoimmune demyelinating disease. Its pathogenesis involves humoral and cellular immunity, with production of pro- and anti-inflammatory cytokines by T cells. To analyze the cytokine profile of cerebrospinal fluid (CSF) T cells in patients with relapsing-remitting MS (RRMS) and non-inflammatory controls. T cell cytokine production was analyzed by flow cytometry in CSF samples collected from 34 untreated RRMS patients and 20 age-matched controls. Immunofluorescence studies were performed in spinal cord MS active lesions. Percentages of CSF-derived IL-17A, IL-17A/IL-22, and IL-17A/GM-CSF producing T cells were significantly higher in RRMS patients compared to controls. Percentages of T cells producing IFN-γ were lower in RRMS patients compared to controls. Patients in relapse showed higher percentages of CD4 We observed differences in IL-17, IL-22, and IFN-γ production by CSF T cells in RRMS versus controls and a positive correlation between IL-13-producing T cells and EDSS in RRMS patients.

Sections du résumé

BACKGROUND
Multiple sclerosis (MS) is a central nervous system (CNS) autoimmune demyelinating disease. Its pathogenesis involves humoral and cellular immunity, with production of pro- and anti-inflammatory cytokines by T cells.
OBJECTIVE
To analyze the cytokine profile of cerebrospinal fluid (CSF) T cells in patients with relapsing-remitting MS (RRMS) and non-inflammatory controls.
METHODS
T cell cytokine production was analyzed by flow cytometry in CSF samples collected from 34 untreated RRMS patients and 20 age-matched controls. Immunofluorescence studies were performed in spinal cord MS active lesions.
RESULTS
Percentages of CSF-derived IL-17A, IL-17A/IL-22, and IL-17A/GM-CSF producing T cells were significantly higher in RRMS patients compared to controls. Percentages of T cells producing IFN-γ were lower in RRMS patients compared to controls. Patients in relapse showed higher percentages of CD4
CONCLUSION
We observed differences in IL-17, IL-22, and IFN-γ production by CSF T cells in RRMS versus controls and a positive correlation between IL-13-producing T cells and EDSS in RRMS patients.

Identifiants

DOI: 10.1177/1352458519852092 PMID: 31237799
pubmed: 31237799
doi: 10.1177/1352458519852092
doi:

Substances chimiques

Interleukin-13 0
Granulocyte-Macrophage Colony-Stimulating Factor 83869-56-1

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1172-1186

Subventions

Organisme : NCRR NIH HHS
ID : C06 RR014513
Pays : United States

Auteurs

Laura Ghezzi (L)

Department of Neurology, School of Medicine, Washington University, St. Louis, MO, USA/Centro Dino Ferrari, University of Milan, Milan, Italy/Fondazione IRCCS Ca' Granda, Ospedale Policlinico, Milan, Italy.

Claudia Cantoni (C)

Department of Neurology, School of Medicine, Washington University, St. Louis, MO, USA.

Francesca Cignarella (F)

Department of Neurology, School of Medicine, Washington University, St. Louis, MO, USA.

Bryan Bollman (B)

Department of Neurology, School of Medicine, Washington University, St. Louis, MO, USA.

Anne H Cross (AH)

Department of Neurology, School of Medicine, Washington University, St. Louis, MO, USA/Hope Center for Neurological Disorders, School of Medicine, Washington University, St. Louis, MO, USA.

Amber Salter (A)

Division of Biostatistics, School of Medicine, Washington University, St. Louis, MO, USA.

Daniela Galimberti (D)

Department of Biomedical, Surgical and Dental Science, University of Milan, Milan, Italy/Centro Dino Ferrari, University of Milan, Milan, Italy/Fondazione IRCCS Ca' Granda, Ospedale Policlinico, Milan, Italy.

Marina Cella (M)

Department of Pathology and Immunology, School of Medicine, Washington University, St. Louis, MO, USA/Hope Center for Neurological Disorders, School of Medicine, Washington University, St. Louis, MO, USA.

Laura Piccio (L)

Department of Neurology, School of Medicine, Washington University, St. Louis, MO, USA/Hope Center for Neurological Disorders, School of Medicine, Washington University, St. Louis, MO, USA/Brain and Mind Centre, University of Sydney, Sydney, New South Wales, Australia.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Facteurs de croissance hématopoïétique › Facteurs de stimulation des colonies › Facteur de stimulation des colonies de granulocytes et de macrophages › T cells producing GM-CSF and IL-13 are...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › T cells producing GM-CSF and IL-13 are...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Interleukines › Interleukine-13 › T cells producing GM-CSF and IL-13 are...
questionsmedicales.fr › Maladies du système immunitaire › Maladies auto-immunes › Maladies auto-immunes du système nerveux › Maladies démyélinisantes auto-immunes du SNC › Sclérose en plaques › T cells producing GM-CSF and IL-13 are...
questionsmedicales.fr › Maladies du système immunitaire › Maladies auto-immunes › Maladies auto-immunes du système nerveux › Maladies démyélinisantes auto-immunes du SNC › Sclérose en plaques › Sclérose en plaques récurrente-rémittente › T cells producing GM-CSF and IL-13 are...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Caractéristiques de la maladie › Récidive › T cells producing GM-CSF and IL-13 are...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › T cells producing GM-CSF and IL-13 are...