HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis.
BRCA2
HSF2BP
homologous recombination
meiosis
spermatogenesis
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
25 06 2019
25 06 2019
Historique:
received:
16
07
2018
revised:
01
05
2019
accepted:
23
05
2019
entrez:
27
6
2019
pubmed:
27
6
2019
medline:
31
7
2020
Statut:
ppublish
Résumé
The tumor suppressor BRCA2 is essential for homologous recombination (HR), replication fork stability, and DNA interstrand crosslink repair in vertebrates. We identify HSF2BP, a protein previously described as testis specific and not characterized functionally, as an interactor of BRCA2 in mouse embryonic stem cells, where the 2 proteins form a constitutive complex. HSF2BP is transcribed in all cultured human cancer cell lines tested and elevated in some tumor samples. Inactivation of the mouse Hsf2bp gene results in male infertility due to a severe HR defect during spermatogenesis. The BRCA2-HSF2BP interaction is highly evolutionarily conserved and maps to armadillo repeats in HSF2BP and a 68-amino acid region between the BRC repeats and the DNA binding domain of human BRCA2 (Gly2270-Thr2337) encoded by exons 12 and 13. This region of BRCA2 does not harbor known cancer-associated missense mutations and may be involved in the reproductive rather than the tumor-suppressing function of BRCA2.
Identifiants
pubmed: 31242413
pii: S2211-1247(19)30735-1
doi: 10.1016/j.celrep.2019.05.096
pii:
doi:
Substances chimiques
BRCA2 Protein
0
BRCA2 protein, human
0
BRCA2 protein, mouse
0
Carrier Proteins
0
HSF2BP protein, mouse
0
Heat-Shock Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3790-3798.e7Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.