Effect of NIR laser therapy by MLS-MiS source against neuropathic pain in rats: in vivo and ex vivo analysis.
Animals
Behavior, Animal
Excitatory Amino Acid Transporter 2
/ metabolism
Hyperalgesia
/ complications
Inflammation
Lasers
Low-Level Light Therapy
/ methods
Male
Myelin Basic Protein
/ metabolism
Myelin Sheath
/ radiation effects
Neuralgia
/ radiotherapy
Nitric Oxide Synthase Type II
/ antagonists & inhibitors
Pain Threshold
Pressure
Quality of Life
Rats
Rats, Sprague-Dawley
Sciatic Nerve
/ injuries
Spinal Cord
/ radiation effects
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
26 06 2019
26 06 2019
Historique:
received:
05
10
2018
accepted:
03
06
2019
entrez:
28
6
2019
pubmed:
28
6
2019
medline:
30
10
2020
Statut:
epublish
Résumé
Neuropathic pain is characterized by an uncertain etiology and by a poor response to common therapies. The ineffectiveness and the frequent side effects of the drugs used to counteract neuropathic pain call for the discovery of new therapeutic strategies. Laser therapy proved to be effective for reducing pain sensitivity thus improving the quality of life. However, its application parameters and efficacy in chronic pain must be further analyzed. We investigated the pain relieving and protective effect of Photobiomodulation Therapy in a rat model of compressive mononeuropathy induced by Chronic Constriction Injury of the sciatic nerve (CCI). Laser (MLS-MiS) applications started 7 days after surgery and were performed ten times over a three week period showing a reduction in mechanical hypersensitivity and spontaneous pain that started from the first laser treatment until the end of the experiment. The ex vivo analysis highlighted the protective role of laser through the myelin sheath recovery in the sciatic nerve, inhibition of iNOS expression and enhancement of EAAT-2 levels in the spinal cord. In conclusion, this study supports laser treatment as a future therapeutic strategy in patients suffering from neuropathic pain induced by trauma.
Identifiants
pubmed: 31243320
doi: 10.1038/s41598-019-45469-5
pii: 10.1038/s41598-019-45469-5
pmc: PMC6594937
doi:
Substances chimiques
Excitatory Amino Acid Transporter 2
0
Myelin Basic Protein
0
Slc1a2 protein, rat
0
Nitric Oxide Synthase Type II
EC 1.14.13.39
Nos2 protein, rat
EC 1.14.13.39
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9297Références
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