Efficient biosynthesis of anticancer polysaccharide by a mutant Chaetomium globosum ALE20 via non-sterilized fermentation.
Adaptive laboratory evolution
Anticancer polysaccharide
Methanol-resistant strain
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Sep 2019
01 Sep 2019
Historique:
received:
25
05
2019
revised:
13
06
2019
accepted:
24
06
2019
pubmed:
30
6
2019
medline:
9
1
2020
entrez:
29
6
2019
Statut:
ppublish
Résumé
The sterilization process, due to its immense energy consumption, high facilities investment, and loss of raw materials by caramelization, during industrial production has drawn much attention. In this study, a methanol-resistant mutant strain, Chaetomium globosum ALE20, was obtained following 20 cycles of adaptive laboratory evolution process. The titer of anticancer polysaccharide (GCP-M) from C. globosum ALE20 reached 9.2 g/L with glycerol as sole carbon source using non-sterilized and fed-batch fermentation strategy. This titer represents a 200% increase compared with the 3.3 g/L attained with batch fermentation. The GCP-M monosaccharide was comprised of galactose, glucose, mannose and glucuronic acid, in a molar ratio of 3.83:66.37:3.26:1.95, respectively, and its weight-average molecular weight and polydispersity were 3.796 × 10
Identifiants
pubmed: 31252005
pii: S0141-8130(19)33886-3
doi: 10.1016/j.ijbiomac.2019.06.186
pii:
doi:
Substances chimiques
Antineoplastic Agents
0
Fungal Proteins
0
Monosaccharides
0
Glycerol
PDC6A3C0OX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1106-1111Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.