Age and cognitive status dependent differences in blood steroid and thyroid hormone concentrations in intact male rats.
Age Factors
Aging
/ physiology
Animals
Cognition
/ physiology
Cognitive Dysfunction
/ physiopathology
Dihydrotestosterone
/ analysis
Estradiol
/ analysis
Hormones
/ analysis
Learning
/ physiology
Male
Rats
Rats, Sprague-Dawley
Steroids
/ analysis
Testosterone
/ analysis
Thyroid Gland
/ metabolism
Thyroid Hormones
/ analysis
Aging
Cognitive decline
Rat
Steroid hormones
Testosterone
Thyroid hormones
Journal
Behavioral and brain functions : BBF
ISSN: 1744-9081
Titre abrégé: Behav Brain Funct
Pays: England
ID NLM: 101245751
Informations de publication
Date de publication:
30 Jun 2019
30 Jun 2019
Historique:
received:
27
01
2019
accepted:
21
06
2019
entrez:
2
7
2019
pubmed:
2
7
2019
medline:
8
1
2020
Statut:
epublish
Résumé
Age-dependent alterations of hormonal states have been considered to be involved in age related decline of cognitive abilities. Most of the studies in animal models are based on hormonal substitution in adrenal- and/or gonadectomized rodents or infusion of steroid hormones in intact rats. Moreover, the manipulations have been done timely, closely related to test procedures, thus reflecting short-term hormonal mechanisms in the regulation of learning and memory. Here we studied whether more general states of steroid and thyroid hormone profiles, independent from acute experiences, may possibly reflect long-term learning capacity. A large cohort of aged (17-18 months) intact male rats were tested in a spatial hole-board learning task and a subset of inferior and superior learners was included into the analysis. Young male adult rats (16 weeks of age) were also tested. Four to 8 weeks after testing blood plasma samples were taken and hormone concentrations of a variety of steroid hormones were measured by gas chromatography-tandem mass spectrometry or radioimmunoassay (17β-estradiol, thyroid hormones). Aged good learners were similar to young rats in the behavioral task. Aged poor learners but not good learners showed higher levels of triiodothyronine (T3) as compared to young rats. Aged good learners had higher levels of thyroid stimulating hormone (TSH) than aged poor learning and young rats. Both aged good and poor learners showed significantly reduced levels of testosterone (T), 4-androstenedione (4A), androstanediol-3α,17β (AD), dihydrotestosterone (DHT), 17-hydroxyprogesterone (17OHP), higher levels of progesterone (Prog) and similar levels of 17β-estradiol (E2) as compared to young rats. The learning, but not the memory indices of all rats were significantly and positively correlated with levels of dihydrotestosterone, androstanediol-3α,17β and thyroxine (T4), when the impacts of age and cognitive division were eliminated by partial correlation analyses. The correlation of hormone concentrations of individuals with individual behavior revealed a possible specific role of these androgen and thyroid hormones in a state of general preparedness to learn.
Sections du résumé
BACKGROUND
BACKGROUND
Age-dependent alterations of hormonal states have been considered to be involved in age related decline of cognitive abilities. Most of the studies in animal models are based on hormonal substitution in adrenal- and/or gonadectomized rodents or infusion of steroid hormones in intact rats. Moreover, the manipulations have been done timely, closely related to test procedures, thus reflecting short-term hormonal mechanisms in the regulation of learning and memory. Here we studied whether more general states of steroid and thyroid hormone profiles, independent from acute experiences, may possibly reflect long-term learning capacity. A large cohort of aged (17-18 months) intact male rats were tested in a spatial hole-board learning task and a subset of inferior and superior learners was included into the analysis. Young male adult rats (16 weeks of age) were also tested. Four to 8 weeks after testing blood plasma samples were taken and hormone concentrations of a variety of steroid hormones were measured by gas chromatography-tandem mass spectrometry or radioimmunoassay (17β-estradiol, thyroid hormones).
RESULTS
RESULTS
Aged good learners were similar to young rats in the behavioral task. Aged poor learners but not good learners showed higher levels of triiodothyronine (T3) as compared to young rats. Aged good learners had higher levels of thyroid stimulating hormone (TSH) than aged poor learning and young rats. Both aged good and poor learners showed significantly reduced levels of testosterone (T), 4-androstenedione (4A), androstanediol-3α,17β (AD), dihydrotestosterone (DHT), 17-hydroxyprogesterone (17OHP), higher levels of progesterone (Prog) and similar levels of 17β-estradiol (E2) as compared to young rats. The learning, but not the memory indices of all rats were significantly and positively correlated with levels of dihydrotestosterone, androstanediol-3α,17β and thyroxine (T4), when the impacts of age and cognitive division were eliminated by partial correlation analyses.
CONCLUSION
CONCLUSIONS
The correlation of hormone concentrations of individuals with individual behavior revealed a possible specific role of these androgen and thyroid hormones in a state of general preparedness to learn.
Identifiants
pubmed: 31256760
doi: 10.1186/s12993-019-0161-3
pii: 10.1186/s12993-019-0161-3
pmc: PMC6600892
doi:
Substances chimiques
Hormones
0
Steroids
0
Thyroid Hormones
0
Dihydrotestosterone
08J2K08A3Y
Testosterone
3XMK78S47O
Estradiol
4TI98Z838E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
10Références
J Androl. 2000 Jan-Feb;21(1):72-84
pubmed: 10670522
Psychoneuroendocrinology. 2001 Oct;26(7):731-50
pubmed: 11500254
Neurosci Biobehav Rev. 2002 Jan;26(1):45-60
pubmed: 11835983
Reprod Domest Anim. 2003 Feb;38(1):13-20
pubmed: 12535324
Ann Endocrinol (Paris). 2003 Apr;64(2):158-61
pubmed: 12773955
Neurobiol Learn Mem. 2004 Jan;81(1):67-74
pubmed: 14670360
Prog Neurobiol. 2003 Dec;71(6):401-37
pubmed: 15013227
J Gerontol. 1992 Sep;47(5):B164-70
pubmed: 1512426
Psychoneuroendocrinology. 2004 Aug;29(7):891-8
pubmed: 15177704
Pharmacol Biochem Behav. 1992 Jan;41(1):145-52
pubmed: 1539064
Endocrinology. 2005 Mar;146(3):1372-81
pubmed: 15564338
Psychoneuroendocrinology. 2005 Jun;30(5):418-30
pubmed: 15721054
Exp Gerontol. 2005 Apr;40(4):330-4
pubmed: 15820614
Mol Cell Neurosci. 2005 Jul;29(3):414-26
pubmed: 15950154
Exp Neurol. 2005 Oct;195(2):330-41
pubmed: 16004982
Aging Male. 2005 Mar;8(1):31-8
pubmed: 16106921
Eur J Cell Biol. 2006 Jul;85(7):635-42
pubmed: 16600423
Pharmacol Biochem Behav. 2007 Feb;86(2):415-21
pubmed: 16828856
Neurobiol Learn Mem. 2007 Sep;88(2):208-16
pubmed: 17507257
Genes Brain Behav. 2007 Jun;6 Suppl 1:40-4
pubmed: 17543038
Steroids. 1976 Feb;27(2):167-85
pubmed: 179167
Mech Ageing Dev. 1991 Dec 31;61(3):275-85
pubmed: 1795566
J Neurosci. 2010 Mar 10;30(10):3813-25
pubmed: 20220016
J Pharmacol Exp Ther. 2010 Sep 1;334(3):1031-41
pubmed: 20551294
Front Aging Neurosci. 2010 Apr 08;2:15
pubmed: 20552051
J Neurosci. 1991 Jul;11(7):2102-6
pubmed: 2066776
Neuropharmacology. 2011 Jun;60(7-8):1254-61
pubmed: 21215272
Horm Behav. 2011 Apr;59(4):581-4
pubmed: 21376723
J Neurosci. 1990 Oct;10(10):3247-54
pubmed: 2170594
Neurobiol Aging. 2012 Aug;33(8):1730-43
pubmed: 21763035
J Neuroendocrinol. 2012 Mar;24(3):422-33
pubmed: 22070634
Neurobiol Aging. 1990 Nov-Dec;11(6):625-30
pubmed: 2280806
Behav Neural Biol. 1990 Jan;53(1):6-20
pubmed: 2302142
Iran Biomed J. 2012;16(3):145-55
pubmed: 23023216
Front Cell Neurosci. 2012 Dec 18;6:40
pubmed: 23264760
J Clin Endocrinol Metab. 2014 Feb;99(2):424-32
pubmed: 24285689
Neurobiol Learn Mem. 2014 Oct;114:101-112
pubmed: 24882624
Behav Brain Res. 2015 Mar 1;280:149-59
pubmed: 25451551
PLoS One. 2015 Feb 23;10(2):e0117795
pubmed: 25706382
Mol Cell Endocrinol. 2015 Sep 5;412:330-8
pubmed: 26021641
Brain Behav Immun. 2015 Oct;49:216-32
pubmed: 26093306
Lancet Diabetes Endocrinol. 2016 Aug;4(8):657-665
pubmed: 27377542
Neuropeptides. 2016 Oct;59:111-116
pubmed: 27640349
JAMA. 2017 Feb 21;317(7):717-727
pubmed: 28241356
Hum Reprod. 2017 Apr 1;32(4):719-724
pubmed: 28333214
J Steroid Biochem Mol Biol. 2018 May;179:88-103
pubmed: 28962971
Lab Anim. 1988 Jul;22(3):276-80
pubmed: 3172710
Horm Metab Res. 1985 Oct;17(10):507-9
pubmed: 4065813
Biochem J. 1966 Apr;99(1):138-45
pubmed: 6007455
Endocr Res Commun. 1982-1983;9(3-4):169-77
pubmed: 6821309
J Steroid Biochem. 1982 Dec;17(6):653-60
pubmed: 6891012
Exp Aging Res. 1981 Spring;7(1):25-32
pubmed: 7238548
Br J Pharmacol. 1977 Mar;59(3):397-402
pubmed: 843682
Exp Gerontol. 1995 Mar-Apr;30(2):169-75
pubmed: 8591810
Eur J Neurosci. 1997 Apr;9(4):637-42
pubmed: 9153570
J Gerontol B Psychol Sci Soc Sci. 1998 Jul;53(4):P234-9
pubmed: 9679515
Nature. 1998 Aug 20;394(6695):787-90
pubmed: 9723618