Cholesterol modulates the pressure response of DMPC membranes.
DMPC/cholesterol membranes
High hydrostatic pressure
Multi-lamellar vesicles
Small angle X-ray scattering
Solid-supported multilayers
X-ray reflectometry
Journal
Biophysical chemistry
ISSN: 1873-4200
Titre abrégé: Biophys Chem
Pays: Netherlands
ID NLM: 0403171
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
06
05
2019
revised:
12
06
2019
accepted:
19
06
2019
pubmed:
3
7
2019
medline:
2
11
2019
entrez:
3
7
2019
Statut:
ppublish
Résumé
In this work, the effect of cholesterol on the pressure response of solid-supported phospholipid multilayers is analyzed. It is shown that DMPC multilayers become highly pressure-responsive by the incorporation of low amounts of cholesterol, resulting in a strong pressure-induced expansion of the bilayer spacing. This is accompanied by a high tendency of the multilayer system to detach from the substrate. Increasing the cholesterol concentration reduces the pressure-induced expansion and the membrane structure remains largely unchanged upon pressurization, consequently the stability of the multilayers improves. For a determination of the influence of the substrate, the pressure-dependent behavior of multilayers is compared to that of solid-supported bilayers and multi-lamellar vesicles in bulk solution. While single-supported bilayers remain largely unaffected by external pressure independent of their cholesterol content, multi-lamellar vesicles and multilayers behave similarly.
Identifiants
pubmed: 31265976
pii: S0301-4622(19)30195-4
doi: 10.1016/j.bpc.2019.106210
pii:
doi:
Substances chimiques
Lipid Bilayers
0
Cholesterol
97C5T2UQ7J
Dimyristoylphosphatidylcholine
U86ZGC74V5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
106210Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.