A genetic system for biasing the sex ratio in mice.
Animals
Breeding
CRISPR-Associated Protein 9
/ genetics
CRISPR-Cas Systems
Chromosomes, Mammalian
Clustered Regularly Interspaced Short Palindromic Repeats
Crosses, Genetic
Female
Fertilization
Gene Editing
/ methods
Genome
Male
Mice
Oocytes
/ cytology
RNA, Guide, Kinetoplastida
/ genetics
Sex Determination Processes
Sex Ratio
Spermatozoa
/ cytology
CRISPR-Cas
Y chromosome
genetic system
mammals
sex ratio
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
11
04
2019
revised:
14
05
2019
accepted:
27
05
2019
pubmed:
4
7
2019
medline:
29
4
2020
entrez:
4
7
2019
Statut:
ppublish
Résumé
Biasing the sex ratio of populations of different organisms, including plants, insects, crustacean, and fish, has been demonstrated by genetic and non-genetic approaches. However, biasing the sex ratio of mammalian populations has not been demonstrated genetically. Here, we provide a first proof of concept for such a genetic system in mammals by crossing two genetically engineered mouse lines. The maternal line encodes a functional Cas9 protein on an autosomal chromosome, whereas the paternal line encodes guide RNAs on the Y chromosome targeting vital mouse genes. After fertilization, the presence of both the Y-encoded guide RNAs from the paternal sperm and the Cas9 protein from the maternal egg targets the vital genes in males. We show that these genes are specifically targeted in males and that this breeding consequently self-destructs solely males. Our results pave the way for a genetic system that allows biased sex production of livestock.
Identifiants
pubmed: 31267640
doi: 10.15252/embr.201948269
pmc: PMC6680165
doi:
Substances chimiques
RNA, Guide
0
CRISPR-Associated Protein 9
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e48269Subventions
Organisme : Ministry of Science and Technology, Israel
ID : 3-14351
Pays : International
Organisme : The Varda and Boaz Dotan Research Center
Pays : International
Organisme : Israel Science Foundation
ID : 268/14
Pays : International
Organisme : Israel Science Foundation
ID : 1416/15
Pays : International
Organisme : Israel Science Foundation
ID : 818/18
Pays : International
Organisme : EC | H2020 | H2020 Priority Excellent Science | H2020 European Research Council (ERC)
ID : 811322
Pays : International
Organisme : EC | FP7 | FP7 Ideas: European Research Council (FP7 Ideas)
ID : 336079
Pays : International
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 The Authors.
Références
Nature. 1991 May 9;351(6322):117-21
pubmed: 2030730
Cell. 2013 May 9;153(4):910-8
pubmed: 23643243
Nat Commun. 2019 Jan 8;10(1):84
pubmed: 30622266
Nature. 2017 Aug 4;548(7666):148
pubmed: 28796219
J Hered. 1988 Mar-Apr;79(2):89-92
pubmed: 3403962
Science. 2015 Jun 12;348(6240):1268-70
pubmed: 25999371
Nat Biotechnol. 2018 Dec;36(11):1062-1066
pubmed: 30247490
Semin Reprod Med. 2012 Oct;30(5):351-63
pubmed: 23044871
Mol Cell Biol. 2007 May;27(9):3481-8
pubmed: 17325031
Genome Biol. 2017 Nov 24;18(1):224
pubmed: 29178945
Nat Biotechnol. 2014 May;32(5):424-7
pubmed: 24811511
Nucleic Acids Res. 2014 Dec 16;42(22):e168
pubmed: 25300484
Animal. 2018 Jun;12(s1):s97-s103
pubmed: 29631644
Mar Biotechnol (NY). 2016 Oct;18(5):554-563
pubmed: 27650072
Science. 2018 May 11;360(6389):645-648
pubmed: 29748283
Genetics. 1966 Jun;53(6):1177-96
pubmed: 5958915
Nature. 1961 Jun 10;190:989-90
pubmed: 13729786
Sci Rep. 2016 Aug 03;6:31139
pubmed: 27484623
Nature. 2011 Mar 17;471(7338):368-72
pubmed: 21368762
Nature. 2016 Sep 22;537(7621):508-514
pubmed: 27626380
Physiol Rev. 2007 Jan;87(1):1-28
pubmed: 17237341
Nat Biotechnol. 2016 Jan;34(1):78-83
pubmed: 26641531
Proc Natl Acad Sci U S A. 2018 May 22;115(21):5474-5479
pubmed: 29735715
Cell. 2014 Oct 9;159(2):440-55
pubmed: 25263330
J Hered. 1975 Jan-Feb;66(1):2-4
pubmed: 1141683
Chromosoma. 1974 May 21;46(1):91-120
pubmed: 4134896
Nat Commun. 2014 Jun 10;5:3977
pubmed: 24915045
Mar Biotechnol (NY). 2013 Jun;15(3):321-8
pubmed: 23053056