The mechanism-based toxicity screening of particles with use in the food and nutrition sector via the ToxTracker reporter system.
Animals
Caco-2 Cells
DNA Damage
Embryonic Stem Cells
/ drug effects
Food Additives
/ toxicity
Food Industry
Gastrointestinal Tract
/ cytology
Glutathione
/ metabolism
Hep G2 Cells
High-Throughput Screening Assays
Humans
Liver
/ cytology
Mice
Nanoparticles
/ toxicity
Oxidative Stress
/ drug effects
Titanium
/ toxicity
Toxicity Tests
/ methods
Comet assay
DNA damage
E153
E171
Oxidative stress
ToxTracker
Journal
Toxicology in vitro : an international journal published in association with BIBRA
ISSN: 1879-3177
Titre abrégé: Toxicol In Vitro
Pays: England
ID NLM: 8712158
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
26
02
2019
revised:
19
06
2019
accepted:
03
07
2019
pubmed:
8
7
2019
medline:
25
3
2020
entrez:
8
7
2019
Statut:
ppublish
Résumé
The rapid expansion of the incorporation of nano-sized materials in consumer products overlaps with the necessity for high-throughput reliable screening tools for the identification of the potential hazardous properties of the nanomaterials. The ToxTracker assay (mechanism-based reporter assay based on embryonic stem cells that uses GFP-tagged biomarkers for detection of DNA damage, oxidative stress and general cellular stress) is one such tool, which could prove useful in the field of particle toxicology allowing for high throughput screening. Here, ToxTracker was utilised to evaluate the potential hazardous properties of two particulates currently used in the food industry (vegetable carbon (E153) and food-grade TiO
Identifiants
pubmed: 31279906
pii: S0887-2333(19)30180-8
doi: 10.1016/j.tiv.2019.104594
pii:
doi:
Substances chimiques
Food Additives
0
titanium dioxide
15FIX9V2JP
Titanium
D1JT611TNE
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104594Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.