Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata.
Arabidopsis
/ cytology
Arabidopsis Proteins
/ genetics
Cell Membrane
/ metabolism
Cells, Cultured
Endoplasmic Reticulum
/ metabolism
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genes, Reporter
Glycosyltransferases
/ deficiency
Green Fluorescent Proteins
/ genetics
Humans
Luminescent Proteins
/ genetics
Membrane Proteins
/ deficiency
Phospholipids
/ metabolism
Plant Cells
Plants, Genetically Modified
Plasmodesmata
/ genetics
Protein Domains
Saccharomyces cerevisiae
/ genetics
Signal Transduction
Nicotiana
/ genetics
Red Fluorescent Protein
ER-PM membrane contact sites
intercellular communication in plants
multiple C2 domains and transmembrane region proteins
plasmodesmata
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
03
10
2018
revised:
28
05
2019
accepted:
06
06
2019
pubmed:
10
7
2019
medline:
29
4
2020
entrez:
10
7
2019
Statut:
ppublish
Résumé
In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplasmic reticulum (ER)-plasma membrane (PM) contacts coincide with regulation of cell-to-cell signalling. The molecular mechanism and function of membrane tethering within plasmodesmata remain unknown. Here, we show that the multiple C2 domains and transmembrane region protein (MCTP) family, key regulators of cell-to-cell signalling in plants, act as ER-PM tethers specifically at plasmodesmata. We report that MCTPs are plasmodesmata proteins that insert into the ER via their transmembrane region while their C2 domains dock to the PM through interaction with anionic phospholipids. A Atmctp3/Atmctp4 loss of function mutant induces plant developmental defects, impaired plasmodesmata function and composition, while MCTP4 expression in a yeast Δtether mutant partially restores ER-PM tethering. Our data suggest that MCTPs are unique membrane tethers controlling both ER-PM contacts and cell-to-cell signalling.
Identifiants
pubmed: 31286648
doi: 10.15252/embr.201847182
pmc: PMC6680132
doi:
Substances chimiques
Arabidopsis Proteins
0
Luminescent Proteins
0
Membrane Proteins
0
Phospholipids
0
Green Fluorescent Proteins
147336-22-9
AT1G51570 protein, Arabidopsis
EC 2.4.-
AT3G57880 protein, Arabidopsis
EC 2.4.-
Glycosyltransferases
EC 2.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e47182Subventions
Organisme : EC|H2020|H2020 Priority Excellent Science|H2020 European Research Council (ERC)
ID : 772103-BRIDGING
Pays : International
Organisme : Centre National de la Recherche Scientifique (CNRS)
ID : OSEZ-2017-BRIDGING
Pays : International
Organisme : Agence Nationale de la Recherche (ANR)
ID : ANR-14-CE19-0006-01
Pays : International
Organisme : FNRS|Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture (FRIA)
ID : 1.E.096.18
Pays : International
Organisme : Fonds De La Recherche Scientifique - FNRS (FNRS)
ID : NEAMEMB PDR T.1003.14
Pays : International
Organisme : Fonds De La Recherche Scientifique - FNRS (FNRS)
ID : BRIDGING CDR J.0114.18
Pays : International
Organisme : Fonds De La Recherche Scientifique - FNRS (FNRS)
ID : RHAMEMB CDR J.0086.18
Pays : International
Organisme : RCUK|Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/M007200/1
Pays : International
Informations de copyright
© 2019 The Authors. Published under the terms of the CC BY 4.0 license.
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