Biophysical characterization and single-chain Fv construction of a neutralizing antibody to measles virus.
Antibodies, Neutralizing
/ biosynthesis
Antibodies, Viral
/ biosynthesis
Epitopes
/ immunology
HEK293 Cells
Humans
Immunoglobulin Fab Fragments
/ biosynthesis
Measles
/ immunology
Measles virus
/ immunology
Nectins
/ antagonists & inhibitors
Protein Binding
Signaling Lymphocytic Activation Molecule Family Member 1
/ antagonists & inhibitors
Single-Chain Antibodies
/ biosynthesis
Viral Proteins
/ immunology
Virus Internalization
SLAM
Measles virus
Nectin-4
hemagglutinin
neutralizing antibody
single-chain fragment variables
surface plasmon resonance
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
02
12
2018
revised:
01
05
2019
accepted:
06
07
2019
pubmed:
10
7
2019
medline:
9
7
2020
entrez:
10
7
2019
Statut:
ppublish
Résumé
The measles virus (MV) is a major cause of childhood morbidity and mortality worldwide. We previously established a mouse monoclonal antibody, 2F4, which shows high neutralizing titers against eight different genotypes of MV. However, the molecular basis for the neutralizing activity of the 2F4 antibody remains incompletely understood. Here, we have evaluated the binding characteristics of a Fab fragment of the 2F4 antibody. Using the MV infectious assay, we demonstrated that 2F4 Fab inhibits viral entry via either of two cellular receptors, SLAM and Nectin4. Surface plasmon resonance (SPR) analysis of recombinant proteins indicated that 2F4 Fab interacts with MV hemagglutinin (MV-H) with a K
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Epitopes
0
Immunoglobulin Fab Fragments
0
NECTIN1 protein, human
0
Nectins
0
SLAMF1 protein, human
0
Single-Chain Antibodies
0
Viral Proteins
0
Signaling Lymphocytic Activation Molecule Family Member 1
169535-43-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
145-159Informations de copyright
© 2019 Federation of European Biochemical Societies.
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