Methylcitrate cycle gene MCD is essential for the virulence of Talaromyces marneffei.
Talaromyces marneffei
methylcitrate dehydratase
the methylcitrate cycle
virulence
Journal
Medical mycology
ISSN: 1460-2709
Titre abrégé: Med Mycol
Pays: England
ID NLM: 9815835
Informations de publication
Date de publication:
01 Apr 2020
01 Apr 2020
Historique:
received:
07
01
2019
revised:
23
04
2019
accepted:
03
07
2019
pubmed:
11
7
2019
medline:
21
10
2020
entrez:
11
7
2019
Statut:
ppublish
Résumé
Talaromyces marneffei (T. marneffei), which used to be known as Penicillium marneffei, is the causative agent of the fatal systemic mycosis known as talaromycosis. For the purpose of understanding the role of methylcitrate cycle in the virulence of T. marneffei, we generated MCD deletion (ΔMCD) and complementation (ΔMCD+) mutants of T. marneffei. Growth in different carbon sources showed that ΔMCD cannot grow on propionate media and grew slowly on the valerate, valine, methionine, isoleucine, cholesterol, and YNB (carbon free) media. The macrophage killing assay showed that ΔMCD was attenuated in macrophages of mice in vitro, especially at the presence of propionate. Finally, virulence studies in a murine infection experiment revealed attenuated virulence of the ΔMCD, which indicates MCD is essential for T. marneffei virulence in the host. This experiment laid the foundation for the further study of the specific mechanisms underlying the methylcitrate cycle of T. marneffei and may provide suitable targets for new antifungals.
Identifiants
pubmed: 31290549
pii: 5530411
doi: 10.1093/mmy/myz063
doi:
Substances chimiques
Culture Media
0
Virulence Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
351-361Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.