HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon.
E. coli
SecA
SecYEG
conformational dynamics
hydrogen-deuterium exchange mass spectromtery
molecular biophysics
protein translocation
structural biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
10 07 2019
10 07 2019
Historique:
received:
04
04
2019
accepted:
09
07
2019
pubmed:
11
7
2019
medline:
23
2
2020
entrez:
11
7
2019
Statut:
epublish
Résumé
The bacterial Sec translocon is a multi-protein complex responsible for translocating diverse proteins across the plasma membrane. For post-translational protein translocation, the Sec-channel - SecYEG - associates with the motor protein SecA to mediate the ATP-dependent transport of pre-proteins across the membrane. Previously, a diffusional-based Brownian ratchet mechanism for protein secretion has been proposed; the structural dynamics required to facilitate this mechanism remain unknown. Here, we employ hydrogen-deuterium exchange mass spectrometry (HDX-MS) to reveal striking nucleotide-dependent conformational changes in the Sec protein-channel from
Identifiants
pubmed: 31290743
doi: 10.7554/eLife.47402
pii: 47402
pmc: PMC6639072
doi:
pii:
Substances chimiques
Escherichia coli Proteins
0
Nucleotides
0
SEC Translocation Channels
0
SecY protein, E coli
0
Adenosine Triphosphate
8L70Q75FXE
SecA protein, E coli
EC 3.6.3.-
SecA Proteins
EC 7.4.2.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/I008675/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M003604/1
Pays : United Kingdom
Organisme : Wellcome
ID : 109854/Z/15/Z
Pays : International
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N015126/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_15031
Pays : United Kingdom
Informations de copyright
© 2019, Ahdash et al.
Déclaration de conflit d'intérêts
ZA, EP, WA, RC, IC, AP No competing interests declared
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