RABiT-II-DCA: A Fully-automated Dicentric Chromosome Assay in Multiwell Plates.
Journal
Radiation research
ISSN: 1938-5404
Titre abrégé: Radiat Res
Pays: United States
ID NLM: 0401245
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
pubmed:
12
7
2019
medline:
18
12
2019
entrez:
12
7
2019
Statut:
ppublish
Résumé
We developed a fully-automated dicentric chromosome assay (DCA) in multiwell plates. All operations, from sample loading to chromosome scoring, are performed, without human intervention, by the second-generation Rapid Automated Biodosimetry Tool II (RABiT-II) robotic system, a plate imager and custom software, FluorQuantDic. The system requires small volumes of blood (30 µl per individual) to determine radiation dose received as a result of a radiation accident or terrorist attack. To visualize dicentrics in multiwell plates, we implemented a non-classical protocol for centromere FISH staining at 37°C. The RABiT-II performs rapid analysis of chromosomes after extracting them from metaphase cells. With the use of multiwell plates, many samples can be screened at the same time. Thus, the RABiT-II DCA provides an advantage during triage when risk-based stratification and medical management are required for a large population exposed to unknown levels of ionizing radiation.
Identifiants
pubmed: 31295087
pii: 10.1667/RR15266.1
doi: 10.1667/RR15266.1
pmc: PMC8567107
mid: NIHMS1652108
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
311-323Subventions
Organisme : NIAID NIH HHS
ID : HHSN272201600040C
Pays : United States
Organisme : NIAID NIH HHS
ID : HHSN272201000040C
Pays : United States
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