Immunomodulatory effects of Rhipicephalus haemaphysaloides serpin RHS2 on host immune responses.
BMDC
Immmunomodulatory
Rhipicephalus haemaphysaloides
Serpin
Tick
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
11 Jul 2019
11 Jul 2019
Historique:
received:
23
12
2018
accepted:
08
07
2019
entrez:
13
7
2019
pubmed:
13
7
2019
medline:
20
8
2019
Statut:
epublish
Résumé
Rhipicephalus haemaphysaloides is a widespread tick species in China and other South East Asian countries, where it is the vector of many pathogens. The objective of this study was to study the role of serpin (serine protease inhibitor) during the tick-host interaction. The differentiation of bone marrow-derived dendritic cells (BMDC) was induced in vitro, and the effect of RHS2 on the maturation of DCs was evaluated. The effects of RHS2 on T cell activation and cytotoxic T lymphocytes' (CTLs) activity were analyzed by flow cytometry. Antibody subtypes after immunization of mice with RHS2 and OVA were determined. RHS2 can inhibit the differentiation of bone marrow-derived cells into DCs and promote their differentiation into macrophages. RHS2 can inhibit the maturation of DCs and the expression of CD80, CD86 and MHCII. The number of CD3 The results show that RHS2 has an inhibitory effect on the host immune response. Ticks have evolved various ways to circumvent adaptive immunity. Their serpin inhibits BMDC differentiation to reduce immune responses.
Sections du résumé
BACKGROUND
BACKGROUND
Rhipicephalus haemaphysaloides is a widespread tick species in China and other South East Asian countries, where it is the vector of many pathogens. The objective of this study was to study the role of serpin (serine protease inhibitor) during the tick-host interaction.
METHODS
METHODS
The differentiation of bone marrow-derived dendritic cells (BMDC) was induced in vitro, and the effect of RHS2 on the maturation of DCs was evaluated. The effects of RHS2 on T cell activation and cytotoxic T lymphocytes' (CTLs) activity were analyzed by flow cytometry. Antibody subtypes after immunization of mice with RHS2 and OVA were determined.
RESULTS
RESULTS
RHS2 can inhibit the differentiation of bone marrow-derived cells into DCs and promote their differentiation into macrophages. RHS2 can inhibit the maturation of DCs and the expression of CD80, CD86 and MHCII. The number of CD3
CONCLUSIONS
CONCLUSIONS
The results show that RHS2 has an inhibitory effect on the host immune response. Ticks have evolved various ways to circumvent adaptive immunity. Their serpin inhibits BMDC differentiation to reduce immune responses.
Identifiants
pubmed: 31296257
doi: 10.1186/s13071-019-3607-4
pii: 10.1186/s13071-019-3607-4
pmc: PMC6624921
doi:
Substances chimiques
Serpins
0
Types de publication
Journal Article
Langues
eng
Pagination
341Subventions
Organisme : National Natural Science Foundation of China
ID : 31572512
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