Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility.
Animals
Chickens
Crystallography, X-Ray
Female
Fertilization
/ physiology
Frameshift Mutation
Humans
Infertility, Female
/ genetics
Molecular Dynamics Simulation
Protein Domains
/ physiology
Protein Multimerization
Recombinant Proteins
/ genetics
Sequence Alignment
Zona Pellucida
/ metabolism
Zona Pellucida Glycoproteins
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
12 07 2019
12 07 2019
Historique:
received:
09
07
2018
accepted:
11
06
2019
entrez:
14
7
2019
pubmed:
14
7
2019
medline:
28
10
2019
Statut:
epublish
Résumé
Mammalian fertilisation begins when sperm interacts with the egg zona pellucida (ZP), whose ZP1 subunit is important for fertility by covalently cross-linking ZP filaments into a three-dimensional matrix. Like ZP4, a structurally-related component absent in the mouse, ZP1 is predicted to contain an N-terminal ZP-N domain of unknown function. Here we report a characterisation of ZP1 proteins carrying mutations from infertile patients, which suggests that, in human, filament cross-linking by ZP1 is crucial to form a stable ZP. We map the function of ZP1 to its ZP-N1 domain and determine crystal structures of ZP-N1 homodimers from a chicken homolog of ZP1. These reveal that ZP filament cross-linking is highly plastic and can be modulated by ZP1 fucosylation and, potentially, zinc sparks. Moreover, we show that ZP4 ZP-N1 forms non-covalent homodimers in chicken but not in human. Together, these data identify human ZP1 cross-links as a promising target for non-hormonal contraception.
Identifiants
pubmed: 31300655
doi: 10.1038/s41467-019-10931-5
pii: 10.1038/s41467-019-10931-5
pmc: PMC6626044
doi:
Substances chimiques
Recombinant Proteins
0
ZP1 protein, human
0
ZP4 protein, human
0
Zona Pellucida Glycoproteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3086Subventions
Organisme : Vetenskapsrådet (Swedish Research Council)
ID : 2016-03999
Pays : International
Organisme : European Molecular Biology Organization (EMBO)
ID : YIP 2009-1931
Pays : International
Organisme : EC | EC Seventh Framework Programm | FP7 Ideas: European Research Council (FP7-IDEAS-ERC - Specific Programme: "Ideas" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013))
ID : 260759
Pays : International
Références
Bioinformatics. 2012 Dec 1;28(23):3150-2
pubmed: 23060610
DNA Seq. 1994;4(6):361-93
pubmed: 7841460
Electrophoresis. 1999 Dec;20(18):3551-67
pubmed: 10612281
Biol Reprod. 2007 Jan;76(1):9-18
pubmed: 17005939
Hum Reprod. 2010 Jul;25(7):1643-56
pubmed: 20504872
Nat Cell Biol. 2002 Jun;4(6):457-61
pubmed: 12021773
Integr Biol (Camb). 2017 Feb 20;9(2):135-144
pubmed: 28102396
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
Curr Top Dev Biol. 2018;130:275-305
pubmed: 29853180
Development. 2001 Apr;128(7):1119-26
pubmed: 11245577
J Cell Biol. 2012 Jun 25;197(7):897-905
pubmed: 22734000
Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):582-601
pubmed: 19465773
Hum Reprod. 2008 Jun;23(6):1385-93
pubmed: 18332087
Biochim Biophys Acta. 1999 Oct 28;1447(2-3):303-6
pubmed: 10542331
Nature. 2008 Dec 4;456(7222):653-7
pubmed: 19052627
J Cell Biol. 2014 Jun 23;205(6):801-9
pubmed: 24934154
ACS Chem Biol. 2011 Jul 15;6(7):716-23
pubmed: 21526836
Dev Biol. 1988 Aug;128(2):376-85
pubmed: 3396765
Science. 2010 Jul 9;329(5988):216-9
pubmed: 20616279
Curr Top Dev Biol. 2018;130:413-442
pubmed: 29853186
Nat Struct Mol Biol. 2015 Nov;22(11):833-4
pubmed: 26581513
J Mol Biol. 1985 Jan 20;181(2):253-64
pubmed: 3845123
Cell. 2017 Jun 15;169(7):1315-1326.e17
pubmed: 28622512
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
J Assist Reprod Genet. 2019 Apr;36(4):741-747
pubmed: 30778819
J Synchrotron Radiat. 2006 May;13(Pt 3):227-38
pubmed: 16645249
J Struct Biol. 2016 Apr;194(1):1-7
pubmed: 26850170
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
pubmed: 20057044
FEBS Open Bio. 2015 May 27;5:454-65
pubmed: 26106520
Histol Histopathol. 1998 Jan;13(1):293-300
pubmed: 9476659
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W327-31
pubmed: 15215404
Fertil Steril. 2017 Jun;107(6):1364-1369
pubmed: 28577617
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W382-8
pubmed: 15980494
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W522-5
pubmed: 17488841
Proc Natl Acad Sci U S A. 2016 Feb 9;113(6):1552-7
pubmed: 26811476
Hum Mutat. 2019 Nov;40(11):2001-2006
pubmed: 31292994
J Biol Chem. 2000 Sep 15;275(37):28866-72
pubmed: 10979984
Biol Reprod. 1996 May;54(5):1032-7
pubmed: 8722623
Biol Reprod. 2014 Nov;91(5):107
pubmed: 25253730
Trends Biochem Sci. 1997 Dec;22(12):488-90
pubmed: 9433130
N Engl J Med. 2014 Mar 27;370(13):1220-6
pubmed: 24670168
Development. 1999 Sep;126(17):3847-55
pubmed: 10433913
Nucleic Acids Res. 2018 Jan 4;46(D1):D493-D496
pubmed: 29040681
Protein Sci. 1993 Apr;2(4):669-70
pubmed: 8518738
Mol Cell Biol. 2006 Nov;26(21):7991-8
pubmed: 17047254
Biochem Biophys Res Commun. 2007 Dec 21;364(3):682-8
pubmed: 17964539
Curr Top Dev Biol. 2018;130:379-411
pubmed: 29853184
Biochem J. 2004 Nov 15;384(Pt 1):191-9
pubmed: 15264999
J Biol Chem. 2003 Sep 5;278(36):34189-202
pubmed: 12799386
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Sci Rep. 2016 Apr 26;6:24737
pubmed: 27113677
Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10037-41
pubmed: 11517324
Proc Natl Acad Sci U S A. 1996 May 28;93(11):5431-6
pubmed: 8643592
Dev Biol. 1980 Apr;76(1):185-202
pubmed: 7380091
Science. 2011 Sep 23;333(6050):1761-4
pubmed: 21852454
Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):352-67
pubmed: 22505256
Cell. 1980 Jul;20(3):873-82
pubmed: 7418009
Biol Reprod. 2001 Mar;64(3):822-30
pubmed: 11207197
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Reproduction. 2012 Dec;144(6):649-60
pubmed: 23028122
Reproduction. 2011 Mar;141(3):313-9
pubmed: 21173071
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
Hum Reprod. 1995 Sep;10(9):2467-71
pubmed: 8530692
Hum Reprod. 2005 May;20(5):1148-52
pubmed: 15760956
Development. 1996 Sep;122(9):2903-10
pubmed: 8787763
Cell. 2010 Oct 29;143(3):404-15
pubmed: 20970175
Nucleic Acids Res. 2011 Jan;39(Database issue):D38-51
pubmed: 21097890
FEBS Lett. 1992 Apr 6;300(3):237-40
pubmed: 1313375
Development. 1995 Jul;121(7):1947-56
pubmed: 7635043
J Chem Theory Comput. 2011 Oct 11;7(10):3162-3180
pubmed: 22125473
Curr Top Dev Biol. 2018;130:307-329
pubmed: 29853181
Biol Reprod. 1981 Dec;25(5):997-1008
pubmed: 7326313
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W473-6
pubmed: 17584791
Trends Dev Biol. 2014;8:65-76
pubmed: 26504367
J Cell Biol. 1993 Dec;123(6 Pt 1):1431-40
pubmed: 8253842
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Bioinformatics. 2007 Aug 1;23(15):1871-4
pubmed: 17510169
Biol Reprod. 2002 Feb;66(2):407-14
pubmed: 11804956
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W5-9
pubmed: 18440982
Nat Methods. 2017 Jan;14(1):71-73
pubmed: 27819658
Hum Reprod. 2004 Jul;19(7):1580-6
pubmed: 15142998
J Biol Chem. 2000 Mar 17;275(11):7731-42
pubmed: 10713085