Increased pain and inflammatory sensitivity in growth hormone-releasing hormone (GHRH) knockout mice.


Journal

Prostaglandins & other lipid mediators
ISSN: 1098-8823
Titre abrégé: Prostaglandins Other Lipid Mediat
Pays: United States
ID NLM: 9808648

Informations de publication

Date de publication:
10 2019
Historique:
received: 15 03 2019
revised: 04 06 2019
accepted: 09 07 2019
pubmed: 14 7 2019
medline: 21 5 2020
entrez: 14 7 2019
Statut: ppublish

Résumé

Growth hormone (GH) and GH-releasing hormone (GHRH), in addition to metabolic and endocrine effects, play a role in the modulation of pain and inflammation. We aimed to elucidate the consequences of GHRH deficiency on acute nociceptive stimulation and on both acute and chronic inflammatory stimuli in a mouse model of GH deficiency. Mice with generalized ablation of the GHRH gene (GHRH knock out, GHRHKO, -/-) were compared to wild type (GHRH +/+) mice. Responsiveness to acute nociceptive stimulation and to acute inflammatory stimulation was evaluated by conventional hot plate apparatus and formalin test, respectively. We also evaluated responsiveness to colonic inflammation induced both in vivo, after dextran sodium sulfate (DSS) treatment, or ex vivo, by incubating colon segments with bacterial lipopolysaccaride (LPS). Macroscopical and histological examinations were performed, prostaglandin (PG) E

Identifiants

pubmed: 31301405
pii: S1098-8823(19)30047-4
doi: 10.1016/j.prostaglandins.2019.106362
pii:
doi:

Substances chimiques

Tumor Necrosis Factor-alpha 0
8-epi-prostaglandin F2alpha 27415-26-5
Growth Hormone-Releasing Hormone 9034-39-3
Dinoprost B7IN85G1HY
Cyclooxygenase 2 EC 1.14.99.1
Dinoprostone K7Q1JQR04M

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

106362

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Sheila Leone (S)

Department of Pharmacy, G. d'Annunzio University, Chieti, Italy.

Annalisa Chiavaroli (A)

Department of Pharmacy, G. d'Annunzio University, Chieti, Italy.

Lucia Recinella (L)

Department of Pharmacy, G. d'Annunzio University, Chieti, Italy. Electronic address: lucia.recinella@unich.it.

Giustino Orlando (G)

Department of Pharmacy, G. d'Annunzio University, Chieti, Italy.

Claudio Ferrante (C)

Department of Pharmacy, G. d'Annunzio University, Chieti, Italy.

Guya Diletta Marconi (GD)

Department of Pharmacy, G. d'Annunzio University, Chieti, Italy.

Irene Gasparo (I)

Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy.

Alessandra Bitto (A)

Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy.

Roberto Salvatori (R)

Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Luigi Brunetti (L)

Department of Pharmacy, G. d'Annunzio University, Chieti, Italy.

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Classifications MeSH