Zscan4c activates endogenous retrovirus MERVL and cleavage embryo genes.
Animals
Chromatin
/ chemistry
Embryo, Mammalian
Endogenous Retroviruses
/ genetics
Enhancer Elements, Genetic
Epigenesis, Genetic
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Ontology
Genome
Histones
/ genetics
Mice
Molecular Sequence Annotation
Mouse Embryonic Stem Cells
/ cytology
Retroelements
Transcription Factors
/ genetics
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
19 09 2019
19 09 2019
Historique:
accepted:
28
06
2019
revised:
24
06
2019
received:
25
02
2019
pubmed:
16
7
2019
medline:
18
12
2019
entrez:
16
7
2019
Statut:
ppublish
Résumé
Endogenous retroviruses (ERVs) contribute to ∼10 percent of the mouse genome. They are often silenced in differentiated somatic cells but differentially expressed at various embryonic developmental stages. A minority of mouse embryonic stem cells (ESCs), like 2-cell cleavage embryos, highly express ERV MERVL. However, the role of ERVs and mechanism of their activation in these cells are still poorly understood. In this study, we investigated the regulation and function of the stage-specific expressed ERVs, with a particular focus on the totipotency marker MT2/MERVL. We show that the transcription factor Zscan4c functions as an activator of MT2/MERVL and 2-cell/4-cell embryo genes. Zinc finger domains of Zscan4c play an important role in this process. In addition, Zscan4c interacts with MT2 and regulates MT2-nearby 2-cell/4-cell genes through promoting enhancer activity of MT2. Furthermore, MT2 activation is accompanied by enhanced H3K4me1, H3K27ac, and H3K14ac deposition on MT2. Zscan4c also interacts with GBAF chromatin remodelling complex through SCAN domain to further activate MT2 enhancer activity. Taken together, we delineate a previously unrecognized regulatory axis that Zscan4c interacts with and activates MT2/MERVL loci and their nearby genes through epigenetic regulation.
Identifiants
pubmed: 31304534
pii: 5531184
doi: 10.1093/nar/gkz594
pmc: PMC7145578
doi:
Substances chimiques
Chromatin
0
Histones
0
Retroelements
0
Transcription Factors
0
Zscan4c protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8485-8501Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
Nat Commun. 2018 Dec 3;9(1):5139
pubmed: 30510198
Nat Commun. 2019 Jan 3;10(1):34
pubmed: 30604769
Genes Dev. 2011 Mar 15;25(6):594-607
pubmed: 21357675
Bioinformatics. 2011 Jun 1;27(11):1571-2
pubmed: 21493656
Nature. 2016 Jun 30;534(7609):652-7
pubmed: 27309802
J Cell Physiol. 2015 Oct;230(10):2337-44
pubmed: 25752831
Cell Rep. 2017 Aug 22;20(8):1936-1949
pubmed: 28834755
Curr Protoc Bioinformatics. 2004 May;Chapter 4:Unit 4.10
pubmed: 18428725
Bioinformatics. 2014 Apr 1;30(7):923-30
pubmed: 24227677
Bioinformatics. 2011 Jun 15;27(12):1696-7
pubmed: 21486936
Nucleic Acids Res. 2016 Jan 4;44(D1):D81-9
pubmed: 26612867
Cell Res. 2018 Feb;28(2):204-220
pubmed: 29313530
Cell Stem Cell. 2011 Jun 3;8(6):676-87
pubmed: 21624812
Chromosoma. 2012 Oct;121(5):489-97
pubmed: 22903800
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Genome Biol. 2015 Jul 23;16:148
pubmed: 26201400
Dev Cell. 2014 Apr 14;29(1):7-19
pubmed: 24735877
Nat Genet. 2017 Oct;49(10):1502-1510
pubmed: 28846101
Cell Stem Cell. 2015 Feb 5;16(2):135-41
pubmed: 25658370
Nat Commun. 2017 Mar 06;8:14648
pubmed: 28262751
Cell Stem Cell. 2015 Sep 3;17(3):273-86
pubmed: 26340527
Nature. 2015 Jun 11;522(7555):240-244
pubmed: 25938714
Nature. 2012 Jul 5;487(7405):57-63
pubmed: 22722858
Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8367-72
pubmed: 26100872
Curr Opin Genet Dev. 2017 Oct;46:194-201
pubmed: 28866476
Nat Commun. 2013;4:1966
pubmed: 23739662
Nat Genet. 2018 Mar;50(3):443-451
pubmed: 29483655
Nature. 2016 Sep 22;537(7621):548-552
pubmed: 27626377
Cell Stem Cell. 2016 Jul 7;19(1):81-94
pubmed: 27151458
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W169-75
pubmed: 17576678
Cell Rep. 2017 Jan 3;18(1):1-11
pubmed: 28052240
Nat Genet. 2017 Jun;49(6):941-945
pubmed: 28459456
Proc Natl Acad Sci U S A. 2011 Apr 5;108(14):5718-23
pubmed: 21427230
Genome Biol. 2012 Oct 03;13(10):R89
pubmed: 23034137
J Virol. 1997 Jul;71(7):5652-7
pubmed: 9188643
Epigenetics Chromatin. 2013 Jun 04;6(1):15
pubmed: 23735015
Genes Dev. 2019 Feb 1;33(3-4):194-208
pubmed: 30692203
PLoS Biol. 2019 Jun 21;17(6):e3000324
pubmed: 31226106
Mol Cell. 2014 Jul 17;55(2):277-90
pubmed: 24981170
Proc Natl Acad Sci U S A. 2014 May 6;111(18):6690-5
pubmed: 24757056
Bioinformatics. 2000 Nov;16(11):1040-1
pubmed: 11159316
Cell Stem Cell. 2019 May 2;24(5):724-735.e5
pubmed: 31006620
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Mob DNA. 2016 Dec 1;7:24
pubmed: 27980689
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Nat Genet. 2006 Apr;38(4):431-40
pubmed: 16518401
Retrovirology. 2015 May 29;12:45
pubmed: 26021318
Mol Nutr Food Res. 2006 Mar;50(3):270-4
pubmed: 16521160
Dev Biol. 2007 Jul 15;307(2):539-50
pubmed: 17553482
Trends Genet. 2017 Nov;33(11):871-881
pubmed: 28935117
Nat Genet. 2017 Jun;49(6):935-940
pubmed: 28459454
Nat Genet. 2017 Jun;49(6):925-934
pubmed: 28459457
Development. 2020 Jan 23;147(2):
pubmed: 31806660
Nature. 2014 Dec 18;516(7531):405-9
pubmed: 25317556
Nat Cell Biol. 2018 May;20(5):620-631
pubmed: 29686265
Nat Biotechnol. 2011 Jan;29(1):24-6
pubmed: 21221095
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W187-91
pubmed: 24799436
Nature. 2015 Jun 11;522(7555):221-5
pubmed: 25896322
BMC Bioinformatics. 2008 Dec 29;9:559
pubmed: 19114008
Nat Commun. 2019 Jan 21;10(1):364
pubmed: 30664750
Nat Protoc. 2013 Dec;8(12):2502-15
pubmed: 24263090
Cell. 2017 Jan 26;168(3):442-459.e20
pubmed: 28111071
Nat Genet. 2018 Jan;50(1):106-119
pubmed: 29255263
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
Nat Genet. 2010 Jul;42(7):631-4
pubmed: 20526341
Nat Genet. 2019 Jun;51(6):947-951
pubmed: 31133747
DNA Res. 2015 Oct;22(5):307-18
pubmed: 26324425
Nature. 2017 Mar 23;543(7646):550-554
pubmed: 28273063
Cell. 2015 Sep 24;163(1):230-45
pubmed: 26365490
Nat Genet. 2014 Jun;46(6):558-66
pubmed: 24777452
Nat Cell Biol. 2007 Dec;9(12):1436-41
pubmed: 17982445
Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17108-13
pubmed: 15563594
Nat Rev Cancer. 2017 Jul;17(7):415-424
pubmed: 28642606
Bioinformatics. 2015 Nov 15;31(22):3593-9
pubmed: 26206304
Cell Rep. 2016 Sep 27;17(1):179-192
pubmed: 27681430
BMC Genomics. 2012 Aug 24;13:424
pubmed: 22920947
EMBO Rep. 2019 May;20(5):
pubmed: 30948459
Nat Genet. 2018 Jan;50(1):73-82
pubmed: 29255264
Cell Mol Life Sci. 2008 Nov;65(21):3383-98
pubmed: 18818872
Sci Rep. 2012;2:208
pubmed: 22355722
Nature. 2010 Apr 8;464(7290):858-63
pubmed: 20336070