Structures of single-layer β-sheet proteins evolved from β-hairpin repeats.
human gut microbiome
protein folding
secreted proteins
single-layer β-sheet proteins
structural genomics
β-hairpin repeats
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
16
05
2019
revised:
08
07
2019
accepted:
08
07
2019
pubmed:
16
7
2019
medline:
6
5
2020
entrez:
16
7
2019
Statut:
ppublish
Résumé
Free-standing single-layer β-sheets are extremely rare in naturally occurring proteins, even though β-sheet motifs are ubiquitous. Here we report the crystal structures of three homologous, single-layer, anti-parallel β-sheet proteins, comprised of three or four twisted β-hairpin repeats. The structures reveal that, in addition to the hydrogen bond network characteristic of β-sheets, additional hydrophobic interactions mediated by small clusters of residues adjacent to the turns likely play a significant role in the structural stability and compensate for the lack of a compact hydrophobic core. These structures enabled identification of a family of secreted proteins that are broadly distributed in bacteria from the human gut microbiome and are putatively involved in the metabolism of complex carbohydrates. A conserved surface patch, rich in solvent-exposed tyrosine residues, was identified on the concave surface of the β-sheet. These new modular single-layer β-sheet proteins may serve as a new model system for studying folding and design of β-rich proteins.
Identifiants
pubmed: 31306512
doi: 10.1002/pro.3683
pmc: PMC6699103
doi:
Substances chimiques
Bacterial Proteins
0
Tyrosine
42HK56048U
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1676-1689Subventions
Organisme : NIGMS NIH HHS
ID : U54 GM094586
Pays : United States
Informations de copyright
© 2019 The Protein Society.
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