Genetic homogeneity of Anopheles maculatus in Indonesia and origin of a novel species present in Central Java.
Anopheles maculatus
Indonesia
Maculatus Group
Malaria
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
15 Jul 2019
15 Jul 2019
Historique:
received:
05
04
2019
accepted:
05
07
2019
entrez:
17
7
2019
pubmed:
17
7
2019
medline:
19
9
2019
Statut:
epublish
Résumé
Anopheles maculatus (s.s.) is an important vector of malaria in Indonesia. Previously it was considered the only member of the Maculatus Group present in Indonesia. A novel species was recently identified in the Kulon Progo District in Central Java. Until recently, few investigations have been conducted looking at An. maculatus genetic diversity in Indonesia, including allopatric island populations. Indonesian An. maculatus (s.l.) samples were collected in several locations in Java, Lesser Sunda Island group, Sumatra and in Kulon Progo (Yogyakarta, central Java) where a novel species has been identified. Samples from a 30-year-old colony of the Kulon Progo population were also included in the analysis. Maximum-likelihood analysis established the phylogenies of the ITS2 (nuclear) and cox1 (mitochondrial) markers. Putative times of separation were based on cox1 genetic distances. Two species of the Maculatus Group are present in Indonesia. The novel sibling species is more closely related to Anopheles dispar than to An. maculatus (s.s.). Anopheles maculatus (s.s.) samples are homogeneous based on the ITS2 sequences. Indonesian samples and An. dispar belong to the same cox1 maternal lineage and differ from all other known members of the Maculatus Group. Divergence time between the different populations found in Java was estimated using an established cox1 mutation rate. A novel species within the Maculatus Group, most closely related to An. dispar, is confirmed present in the Kulon Progo area of Central Java. The divergence of this species from An. maculatus (s.s.) is explained by the stable refugia in the Kulon Progo area during the quaternary period of intense volcanic activity throughout most of Java. This novel species awaits detailed morphological description before applying a formal species name. For the interim, it is proposed that the Kulon Progo population be designated An. maculatus var. menoreh to distinguish it from An. maculatus (s.s.).
Sections du résumé
BACKGROUND
BACKGROUND
Anopheles maculatus (s.s.) is an important vector of malaria in Indonesia. Previously it was considered the only member of the Maculatus Group present in Indonesia. A novel species was recently identified in the Kulon Progo District in Central Java. Until recently, few investigations have been conducted looking at An. maculatus genetic diversity in Indonesia, including allopatric island populations.
METHODS
METHODS
Indonesian An. maculatus (s.l.) samples were collected in several locations in Java, Lesser Sunda Island group, Sumatra and in Kulon Progo (Yogyakarta, central Java) where a novel species has been identified. Samples from a 30-year-old colony of the Kulon Progo population were also included in the analysis. Maximum-likelihood analysis established the phylogenies of the ITS2 (nuclear) and cox1 (mitochondrial) markers. Putative times of separation were based on cox1 genetic distances.
RESULTS
RESULTS
Two species of the Maculatus Group are present in Indonesia. The novel sibling species is more closely related to Anopheles dispar than to An. maculatus (s.s.). Anopheles maculatus (s.s.) samples are homogeneous based on the ITS2 sequences. Indonesian samples and An. dispar belong to the same cox1 maternal lineage and differ from all other known members of the Maculatus Group. Divergence time between the different populations found in Java was estimated using an established cox1 mutation rate.
CONCLUSIONS
CONCLUSIONS
A novel species within the Maculatus Group, most closely related to An. dispar, is confirmed present in the Kulon Progo area of Central Java. The divergence of this species from An. maculatus (s.s.) is explained by the stable refugia in the Kulon Progo area during the quaternary period of intense volcanic activity throughout most of Java. This novel species awaits detailed morphological description before applying a formal species name. For the interim, it is proposed that the Kulon Progo population be designated An. maculatus var. menoreh to distinguish it from An. maculatus (s.s.).
Identifiants
pubmed: 31307517
doi: 10.1186/s13071-019-3598-1
pii: 10.1186/s13071-019-3598-1
pmc: PMC6631912
doi:
Substances chimiques
DNA, Intergenic
0
Cyclooxygenase 1
EC 1.14.99.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
351Références
Am J Trop Med Hyg. 1999 Mar;60(3):508-15
pubmed: 10466986
Insect Mol Biol. 1999 Aug;8(3):381-90
pubmed: 10469255
Science. 2000 May 12;288(5468):1033-5
pubmed: 10807570
J Med Entomol. 2000 Nov;37(6):933-7
pubmed: 11126553
Am J Trop Med Hyg. 2002 Mar;66(3):287-92
pubmed: 12139222
J Am Mosq Control Assoc. 2002 Dec;18(4):359-63
pubmed: 12542195
Trends Genet. 2003 Jul;19(7):370-5
pubmed: 12850441
Indian J Malariol. 1957 Dec;11(4):321-38
pubmed: 13548995
Mol Phylogenet Evol. 2003 Dec;29(3):417-34
pubmed: 14615184
J Med Entomol. 2004 May;41(3):287-95
pubmed: 15185927
Med Vet Entomol. 2005 Jun;19(2):158-99
pubmed: 15958025
Infect Genet Evol. 2007 Jan;7(1):93-102
pubmed: 16782411
Acta Trop. 2006 Oct;99(2-3):272-80
pubmed: 17052677
Southeast Asian J Trop Med Public Health. 2006;37 Suppl 2:1-128
pubmed: 17262930
J Med Entomol. 2007 Sep;44(5):723-31
pubmed: 17915501
Infect Genet Evol. 2008 Jul;8(4):489-503
pubmed: 18178531
Mol Phylogenet Evol. 2009 Sep;52(3):588-601
pubmed: 19603555
Mol Biol Evol. 2010 Feb;27(2):221-4
pubmed: 19854763
Mol Ecol. 2010 Jun;19(11):2269-85
pubmed: 20444081
Heredity (Edinb). 2011 Feb;106(2):241-52
pubmed: 20517346
Trans R Soc Trop Med Hyg. 1991 Jan-Feb;85(1):35-6
pubmed: 2068752
Malar J. 2010 Sep 16;9:259
pubmed: 20846447
Parasit Vectors. 2011 May 25;4:89
pubmed: 21612587
Parasitology. 2011 Nov;138(13):1723-9
pubmed: 21679487
Adv Parasitol. 2013;83:173-266
pubmed: 23876873
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
Nat Commun. 2014 Jun 25;5:4248
pubmed: 24963649
Parasit Vectors. 2014 Jul 09;7:316
pubmed: 25008314
Science. 2015 Jan 2;347(6217):1258524
pubmed: 25431491
Springerplus. 2014 Nov 23;3:684
pubmed: 25512885
Trends Parasitol. 2015 Mar;31(3):109-19
pubmed: 25697632
Sci Rep. 2017 Dec 11;7(1):17316
pubmed: 29229945
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Parasit Vectors. 2019 Mar 14;12(1):107
pubmed: 30871633
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):6885-8
pubmed: 8041714
Insect Mol Biol. 1993;2(4):247-57
pubmed: 9087562
Insect Mol Biol. 1997 May;6(2):143-50
pubmed: 9099578
Med Vet Entomol. 1997 Apr;11(2):134-8
pubmed: 9226641