Diazonium-Modified Screen-Printed Electrodes for Immunosensing Growth Hormone in Blood Samples.
diazonium grafting
electrochemical impedance spectroscopy
growth hormone
immunosensor
real samples
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
17 Jul 2019
17 Jul 2019
Historique:
received:
01
06
2019
revised:
11
07
2019
accepted:
15
07
2019
entrez:
20
7
2019
pubmed:
20
7
2019
medline:
24
12
2019
Statut:
epublish
Résumé
Altered growth hormone (GH) levels represent a major global health challenge that would benefit from advances in screening methods that are rapid and low cost. Here, we present a miniaturized immunosensor using disposable screen-printed carbon electrodes (SPCEs) for the detection of GH with high sensitivity. The diazonium-based linker layer was electrochemically deposited onto SPCE surfaces, and subsequently activated using covalent agents to immobilize monoclonal anti-GH antibodies as the sensing layer. The surface modifications were monitored using contact angle measurements and X-ray photoelectron spectroscopy (XPS). The dissociation constant, K
Identifiants
pubmed: 31319551
pii: bios9030088
doi: 10.3390/bios9030088
pmc: PMC6784379
pii:
doi:
Substances chimiques
Diazonium Compounds
0
Growth Hormone
9002-72-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Canada Research Chairs
ID : 950-231116
Organisme : Natural Sciences and Engineering Research Council of Canada
ID : 3655
Organisme : Ontario Ministry of Research, Innovation and Science
ID : 35272
Organisme : Canada Foundation for Innovation
ID : 35272
Références
Endocrinology. 2004 Jun;145(6):2666-79
pubmed: 15033913
Anal Chem. 2010 Aug 1;82(15):6401-8
pubmed: 20669994
Analyst. 2013 Apr 7;138(7):2023-31
pubmed: 23400028
Horm Res. 1999;51 Suppl 1:13-9
pubmed: 10393486
Acta Paediatr Scand Suppl. 1990;370:56-61; discussion 62
pubmed: 2260459
Biosensors (Basel). 2017 Nov 17;7(4):null
pubmed: 29149074
Analyst. 2013 Aug 7;138(15):4378-84
pubmed: 23736898
J Vis Exp. 2016 Jan 07;(107):null
pubmed: 26780354
Anal Chem. 2011 Dec 15;83(24):9306-13
pubmed: 22022860
Anal Bioanal Chem. 2012 May;403(4):939-46
pubmed: 22286079
ACS Nano. 2010 Jul 27;4(7):3817-22
pubmed: 20507175
Anal Chem. 2012 Apr 17;84(8):3485-8
pubmed: 22424137
J Am Chem Soc. 2009 Sep 9;131(35):12497-515
pubmed: 19689159
J Clin Endocrinol Metab. 1988 Jan;66(1):68-71
pubmed: 3335610
Growth Horm IGF Res. 2007 Jun;17(3):220-6
pubmed: 17339122
Asian J Androl. 2008 May;10(3):416-25
pubmed: 18385903
Mol Pharm. 2012 Jul 2;9(7):2039-48
pubmed: 22663348
J Biophotonics. 2013 May;6(5):457-67
pubmed: 22829397
Growth Horm IGF Res. 2009 Aug;19(4):383-7
pubmed: 19467616
Anal Chem. 2011 Apr 15;83(8):3042-9
pubmed: 21417322
Exp Physiol. 2008 Nov;93(11):1157-69
pubmed: 18617577
Langmuir. 2012 May 29;28(21):8072-83
pubmed: 22551237
Talanta. 2013 Sep 30;114:268-75
pubmed: 23953470
Nanoscale. 2012 Dec 7;4(23):7593-9
pubmed: 23108911
ACS Chem Biol. 2010 Nov 19;5(11):1075-86
pubmed: 20825209
Langmuir. 2010 Mar 16;26(6):4320-6
pubmed: 19899797
Langmuir. 2006 Aug 15;22(17):7421-30
pubmed: 16893248
Langmuir. 2008 Jun 17;24(12):6351-8
pubmed: 18473443
Biosens Bioelectron. 2012 Oct-Dec;38(1):308-13
pubmed: 22789151
Biosens Bioelectron. 2012 Jun-Jul;36(1):48-55
pubmed: 22541890
Analyst. 2013 Mar 7;138(5):1427-33
pubmed: 23337885
Anal Chem. 2012 Dec 4;84(23):10252-9
pubmed: 23101768
Clin Chim Acta. 2009 May;403(1-2):56-62
pubmed: 19361471
Br J Pharmacol. 2008 Jun;154(3):542-56
pubmed: 18376417
ACS Nano. 2012 Jun 26;6(6):5549-58
pubmed: 22650249
J Biophotonics. 2017 Jan;10(1):61-67
pubmed: 27669684
Sci Rep. 2017 Sep 8;7(1):10969
pubmed: 28887532
Langmuir. 2011 Apr 5;27(7):4176-83
pubmed: 21348487
Biosens Bioelectron. 2013 Jun 15;44:77-84
pubmed: 23395726
J Am Chem Soc. 2012 Sep 12;134(36):15122-9
pubmed: 22891619
Chem Soc Rev. 2011 May;40(5):2704-18
pubmed: 21290036
Talanta. 2009 May 15;78(3):1011-6
pubmed: 19269465
Sci Rep. 2015 Aug 18;5:13181
pubmed: 26282187
Endocrinology. 1986 Oct;119(4):1497-501
pubmed: 3530720
J Clin Endocrinol Metab. 1991 Feb;72(2):387-91
pubmed: 1991809
Biosens Bioelectron. 2009 Oct 15;25(2):395-9
pubmed: 19692224