Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 and angiopoietin-like protein 4 are associated with the increase of lipoprotein lipase activity in epicardial adipose tissue from diabetic patients.
Adiposity
Aged
Angiopoietin-Like Protein 4
/ analysis
Case-Control Studies
Diabetes Mellitus, Type 2
/ diagnosis
Enzyme Activation
Fatty Acids
/ blood
Female
Humans
Intra-Abdominal Fat
/ enzymology
Lipoprotein Lipase
/ analysis
Lipoproteins, VLDL
/ blood
Male
Middle Aged
PPAR gamma
/ metabolism
Pericardium
Receptors, LDL
/ analysis
Receptors, Lipoprotein
/ analysis
Triglycerides
/ blood
Angiopoietin-like protein 4
Coronary artery disease
Epicardial adipose tissue
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1
Lipoprotein lipase
Journal
Atherosclerosis
ISSN: 1879-1484
Titre abrégé: Atherosclerosis
Pays: Ireland
ID NLM: 0242543
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
14
03
2019
revised:
27
05
2019
accepted:
27
06
2019
pubmed:
20
7
2019
medline:
29
7
2020
entrez:
20
7
2019
Statut:
ppublish
Résumé
Epicardial adipose tissue (EAT) is a visceral AT, surrounding myocardium and coronary arteries. Its volume is higher in Type 2 diabetic (DM2) patients, associated with cardiovascular disease risk. Lipoprotein lipase (LPL) hydrolyses triglycerides (TG) from circulating lipoproteins, supplying fatty acids to AT, contributing to its expansion. We aimed to evaluate LPL expression and activity in EAT from DM2 and no DM2 patients, and its regulators ANGPTL4, GPIHBP1 and PPARγ levels, together with VLDLR expression and EAT LPL association with VLDL characteristics. We studied patients undergoing coronary by-pass graft (CABG) divided into CABG-DM2 (n = 21) and CABG-noDM2 (n = 29), and patients without CABG (No CABG, n = 30). During surgery, EAT and subcutaneous AT (SAT) were obtained, in which LPL activity, gene and protein expression, its regulators and VLDLR protein levels were determined. Isolated circulating VLDLs were characterized. EAT LPL activity was higher in CABG-DM2 compared to CABG-noDM2 and No CABG (p=0.002 and p<0.001) and in CABG-noDM2 compared to No CABG (p=0.02), without differences in its expression. ANGPTL4 levels were higher in EAT from No CABG compared to CABG-DM2 and CABG-noDM2 (p<0.001). GPIHBP1 levels were higher in EAT from CABG-DM2 and CABG-noDM2 compared to No CABG (p= 0.04). EAT from CABG-DM2 presented higher PPARγ levels than CABG-noDM2 and No CABG (p=0.02 and p=0.03). No differences were observed in VLDL composition between groups, although EAT LPL activity was inversely associated with VLDL-TG and TG/protein index (p<0.05). EAT LPL regulation would be mainly post-translational. The higher LPL activity in DM2 could be partly responsible for the increase in EAT volume.
Sections du résumé
BACKGROUND AND AIMS
Epicardial adipose tissue (EAT) is a visceral AT, surrounding myocardium and coronary arteries. Its volume is higher in Type 2 diabetic (DM2) patients, associated with cardiovascular disease risk. Lipoprotein lipase (LPL) hydrolyses triglycerides (TG) from circulating lipoproteins, supplying fatty acids to AT, contributing to its expansion. We aimed to evaluate LPL expression and activity in EAT from DM2 and no DM2 patients, and its regulators ANGPTL4, GPIHBP1 and PPARγ levels, together with VLDLR expression and EAT LPL association with VLDL characteristics.
METHODS
We studied patients undergoing coronary by-pass graft (CABG) divided into CABG-DM2 (n = 21) and CABG-noDM2 (n = 29), and patients without CABG (No CABG, n = 30). During surgery, EAT and subcutaneous AT (SAT) were obtained, in which LPL activity, gene and protein expression, its regulators and VLDLR protein levels were determined. Isolated circulating VLDLs were characterized.
RESULTS
EAT LPL activity was higher in CABG-DM2 compared to CABG-noDM2 and No CABG (p=0.002 and p<0.001) and in CABG-noDM2 compared to No CABG (p=0.02), without differences in its expression. ANGPTL4 levels were higher in EAT from No CABG compared to CABG-DM2 and CABG-noDM2 (p<0.001). GPIHBP1 levels were higher in EAT from CABG-DM2 and CABG-noDM2 compared to No CABG (p= 0.04). EAT from CABG-DM2 presented higher PPARγ levels than CABG-noDM2 and No CABG (p=0.02 and p=0.03). No differences were observed in VLDL composition between groups, although EAT LPL activity was inversely associated with VLDL-TG and TG/protein index (p<0.05).
CONCLUSIONS
EAT LPL regulation would be mainly post-translational. The higher LPL activity in DM2 could be partly responsible for the increase in EAT volume.
Identifiants
pubmed: 31323462
pii: S0021-9150(19)31387-5
doi: 10.1016/j.atherosclerosis.2019.06.915
pii:
doi:
Substances chimiques
ANGPTL4 protein, human
0
Angiopoietin-Like Protein 4
0
Fatty Acids
0
GPIHBP1 protein, human
0
Lipoproteins, VLDL
0
PPAR gamma
0
PPARG protein, human
0
Receptors, LDL
0
Receptors, Lipoprotein
0
Triglycerides
0
VLDL receptor
0
very low density lipoprotein triglyceride
0
LPL protein, human
EC 3.1.1.34
Lipoprotein Lipase
EC 3.1.1.34
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
51-59Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.