An ultra-rapid drug screening method for acetaminophen in blood serum based on probe electrospray ionization-tandem mass spectrometry.
Acetaminophen
Blood serum
Probe electrospray ionization
Rapid drug screening
Tandem mass spectrometry
Journal
Journal of food and drug analysis
ISSN: 2224-6614
Titre abrégé: J Food Drug Anal
Pays: China (Republic : 1949- )
ID NLM: 101630927
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
03
09
2018
revised:
30
01
2019
accepted:
01
02
2019
entrez:
21
7
2019
pubmed:
22
7
2019
medline:
8
7
2020
Statut:
ppublish
Résumé
Poisoning incidents caused by drugs, accidental ingestion of poisons, attempted suicide, homicide, and exposure to toxic compounds occur frequently every year across the globe. This raises the need to rapidly identify toxic agents in poisoned patients in a clinical emergency setting. In addition, determining drug/poison concentration is undoubtedly necessary to arrive at a toxicological treatment plan. The purpose of this study was to develop an ultra-rapid drug screening method for the clinical treatment of poisoning. Probe electrospray ionization (PESI), one of the ambient ionization techniques, is able to detect compounds from various biological materials almost directly. We applied the PESI technique to the rapid detection of acetaminophen (APAP). Blood serum samples were diluted 100-fold with 10 mM ammonium formate/ethanol (1:1 v/v) solution including deuterium-labeled internal standards (IS; APAP-d4). Only 10 μL of the diluted sample was used for measurement. The tandem mass spectrometer (MS/MS) equipped with a PESI was used in selected reaction monitoring mode for the quantitation of APAP; the measurement time was only 18 s. Transitions were set at 152 > 110 for quantitation, 152 > 65 for qualifier, and 156 > 114 for IS (APAP-d4). All measurements were conducted in positive mode. The calibration curve (1/x
Identifiants
pubmed: 31324294
pii: j.jfda.2019.02.001
doi: 10.1016/j.jfda.2019.02.001
pmc: PMC9307038
doi:
Substances chimiques
Analgesics, Non-Narcotic
0
Acetaminophen
362O9ITL9D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
786-792Informations de copyright
Copyright © 2019. Published by Elsevier Taiwan LLC.
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