Oral supplementation with p-coumaric acid protects mice against diabetes-associated spontaneous destruction of periodontal tissue.
Administration, Oral
Animals
Antioxidants
/ metabolism
Cells, Cultured
Coumaric Acids
Diabetes Mellitus, Experimental
/ complications
Dietary Supplements
Fibroblasts
Humans
Male
Mice
Mice, Inbred C57BL
Oxidative Stress
Periodontal Diseases
/ drug therapy
Periodontal Ligament
/ cytology
Propionates
/ pharmacology
Streptozocin
ROS accumulation
anti-inflammation
anti-osteoclastogenesis
coumaric acid
diabetes
spontaneous periodontal destruction
Journal
Journal of periodontal research
ISSN: 1600-0765
Titre abrégé: J Periodontal Res
Pays: United States
ID NLM: 0055107
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
25
11
2018
revised:
09
04
2019
accepted:
09
06
2019
pubmed:
23
7
2019
medline:
16
1
2020
entrez:
23
7
2019
Statut:
ppublish
Résumé
Dietary bioactive materials having anti-inflammatory and antioxidant potentials are able to inhibit diabetes-associated periodontal complications. Although numerous studies indicate that administration of p-coumaric acid (p-CA) ameliorates diabetes and diabetes-related complications, the roles of p-CA on periodontal tissue destruction in diabetic mice and the possible mechanisms therein are not completely understood. In this study, we evaluated whether supplementation with p-CA protects mice against diabetes-associated spontaneous periodontal destruction and also explored the associated mechanism therein using in vivo and in vitro experimental systems. C57BL/6 male mice were divided into sham, streptozotocin (STZ), and STZ+CA groups (n = 5/group). Sham group was intraperitoneally injected with sodium buffer, whereas other two groups were injected with the buffer containing 160 mg/kg of STZ. STZ-induced diabetic mice received oral gavage with p-CA (50 mg/kg) (STZ+CA group) or with buffer only (STZ group) daily for 6 weeks. The effect of p-CA on diabetes-associated spontaneous periodontal destruction was evaluated using μCT analysis, hematoxylin and eosin staining, tartrate-resistant acid phosphatase staining, and immunohistochemical staining methods. The efficacies of p-CA on cell proliferation, osteoblast differentiation, reactive oxygen species (ROS) accumulation, and antioxidant-related marker expression were examined using human periodontal ligament fibroblasts (hPLFs) cultured under high glucose condition. Streptozotocin group exhibited periodontal tissue destruction along with increased inflammation, oxidative stress, and osteoclast formation, as well as with decreased osteogenesis. However, oral administration with p-CA protected mice against STZ-induced periodontal destruction by inhibiting inflammation and osteoclastic activation. STZ+CA group also showed higher expression of antioxidant and osteogenic markers in periodontal tissue than did STZ group. Treatment with high glucose concentration (30 mmol/L) impaired proliferation and osteoblast differentiation of hPLFs along with cellular ROS accumulation, whereas these impairments were almost completely disappeared by supplementation with p-CA. These findings demonstrate that supplementation with p-CA inhibits diabetes-associated spontaneous destruction of periodontal tissue by enhancing anti-inflammatory, anti-osteoclastogenic, and antioxidant defense systems in STZ-treated mice.
Substances chimiques
Antioxidants
0
Coumaric Acids
0
Propionates
0
Streptozocin
5W494URQ81
p-coumaric acid
IBS9D1EU3J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
690-701Subventions
Organisme : Biomedical Research Institute of Chonbuk National University Hospital
ID : CHU2017-0045
Organisme : Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT and future Planning
ID : 2016R1A2A2A05921669
Organisme : Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT and future Planning
ID : 2017R1A2B4009269
Organisme : Ministry of Education
ID : 2018R1D1A1B07047162
Organisme : Ministry of Education
ID : 2018R1D1A1B07046563
Organisme : RDA, Ministry of Agriculture and Forestry
ID : PJ01339402
Informations de copyright
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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