Simultaneous separation and determination of hirsutine and hirsuteine by cyclodextrin-modified micellar electrokinetic capillary chromatography.
cyclodextrin-modified micellar electrokinetic capillary chromatography
hirsuteine
hirsutine
simultaneous determination
Journal
Phytochemical analysis : PCA
ISSN: 1099-1565
Titre abrégé: Phytochem Anal
Pays: England
ID NLM: 9200492
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
13
03
2019
revised:
26
05
2019
accepted:
03
06
2019
pubmed:
23
7
2019
medline:
23
2
2020
entrez:
23
7
2019
Statut:
ppublish
Résumé
Hirsutine and hirsuteine are the main pharmacological activity ingredients of Uncaria rhynchophylla (UR), playing an important role in treating mental and cardiovascular diseases, such as Alzheimer's disease, hypertension, Parkinson's disease, potential anti-cancer activities and so on. To develop a cyclodextrin-modified micellar electrokinetic capillary chromatography (CD-MEKC) method for the simultaneous separation and determination of hirsutine and hirsuteine from UR and its formulations. The optimal method was developed by investigating influences of significant factors on the separation, and this method was successfully applied for the determination of hirsutine and hirsuteine in UR and its formulations. The optimal background electrolyte (BGE) consisted of 40 mM sodium dihydrogen phosphate (pH 7.0), 150 mM 2,6-dimethyl-β-cyclodextrin (DM-β-CD), 3 mM mono-(6-ethylenediamine-6-deoxy)-β-cyclodextrin (ED-β-CD), and 30 mM sodium cholate (SC). Under these conditions, hirsutine and hirsuteine were successfully separated within 13 min at the separation voltage of 15 kV, temperature of 25°C and the detection wavelength of 224 nm. For the analytes, linear calibration curves were performed within the range 5.0-160.0 μg/mL. The limit of detection (LOD, S/N = 3) and the limit of quantitation (LOQ, S/N = 10) were 0.41, 1.42 μg/mL for hirsutine and 0.60, 2.17 μg/mL for hirsuteine, respectively. The recoveries of three samples were from 97.9% to 102.3%. The method was successfully applied to the determination of hirsutine and hirsuteine in UR and its formulations. Meanwhile, it provides an effective reference of the quality control of UR and its formulations.
Substances chimiques
Alkaloids
0
Cyclodextrins
0
hirsuteine
18904-54-6
hirsutine
W596OF93C7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112-118Subventions
Organisme : Fund for Shanxi Key Subject Construction
ID : FSKSC2016
Organisme : Natural Science Foundation of Shanxi Province
ID : 201701D121023
Organisme : Natural Science Foundation of Shanxi Province
ID : 201701D121141
Informations de copyright
© 2019 John Wiley & Sons, Ltd.
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