An Ultrastructural Study of the Extruded Polar Tube of Anncaliia algerae (Microsporidia).
3D-reconstruction
cargo
cryogenic transmission electron microscopy
plunge freeze
polar filament
polar tube protein
spore germination
sporoplasm
Journal
The Journal of eukaryotic microbiology
ISSN: 1550-7408
Titre abrégé: J Eukaryot Microbiol
Pays: United States
ID NLM: 9306405
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
02
05
2019
accepted:
05
07
2019
pubmed:
25
7
2019
medline:
12
9
2020
entrez:
24
7
2019
Statut:
ppublish
Résumé
All microsporidia share a unique, extracellular spore stage, containing the infective sporoplasm and the apparatus for initiating infection. The polar filament/polar tube when exiting the spore transports the sporoplasm through it into a host cell. While universal, these structures and processes have been enigmatic. This study utilized several types of microscopy, describing and extending our understanding of these structures and their functions. Cryogenically preserved polar tubes vary in diameter from 155 to over 200 nm, noticeably larger than fixed-sectioned or negatively stained samples. The polar tube surface is pleated and covered with fine fibrillar material that projects from the surface and is organized in clusters or tufts. These fibrils may be the sites of glycoproteins providing protection and aiding infectivity. The polar tube surface is ridged with 5-6 nm spacing between ridges, enabling the polar tube to rapidly increase its diameter to facilitate the passage of the various cargo including cylinders, sacs or vesicles filled with particulate material and the intact sporoplasm containing a diplokaryon. The lumen of the tube is lined with a membrane that facilitates this passage. Careful examination of the terminus of the tube indicates that it has a closed tip where the membranes for the terminal sac are located.
Identifiants
pubmed: 31332877
doi: 10.1111/jeu.12751
pmc: PMC6944765
mid: NIHMS1042754
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
28-44Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103310
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI124753
Pays : United States
Organisme : NCRR NIH HHS
ID : P41 RR001209
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI091985
Pays : United States
Organisme : NIH HHS
ID : S10 OD016214
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA013330
Pays : United States
Informations de copyright
© 2019 International Society of Protistologists.
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