Investigation of antioxidative effects of a cardioprotective solution in heart tissue.
Alpha-ketoglutarate (α-KG)
Carbonyl proteins
HPLC/UV–Vis
Ischemia reperfusion
Malondialdehyde
Oxidative stress
Journal
Molecular and cellular biochemistry
ISSN: 1573-4919
Titre abrégé: Mol Cell Biochem
Pays: Netherlands
ID NLM: 0364456
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
18
01
2019
accepted:
15
07
2019
pubmed:
26
7
2019
medline:
9
1
2020
entrez:
26
7
2019
Statut:
ppublish
Résumé
A multi-component solution, containing α-ketoglutaric acid (α-KG), 5-hydroxymethylfurfural (5-HMF), N-acetyl-seleno-L-methionine (NASeLM), and N-acetyl-L-methionine (NALM) as active ingredients, has been tested considering its supposed antioxidative effect with respect to heart transplantations. Oxidative stress was induced on isolated rat hearts through occlusion of a coronary artery and in chicken heart tissue through hydrogen peroxide. Both heart types were analyzed and the oxidative stress markers malondialdehyde (MDA) and carbonyl proteins (CPs) were determined via HPLC/UV-Vis. In both approaches, it was found that treatment with the multi-component solution led to a lower amount of MDA and CPs compared to a negative control treated with Krebs-Ringer solution (KRS). Further investigation on chicken heart tissue identified α-KG as antioxidative component in these experiments. However, numerous factors like arrhythmia, vessel dilatation, and minimization of oxidative stress effects play an important role for successful transplantation. Therefore, the investigated multi-component solution might be a novel approach against oxidative stress situations, for example at ischemia reperfusion injury during heart transplantations.
Identifiants
pubmed: 31342300
doi: 10.1007/s11010-019-03591-y
pii: 10.1007/s11010-019-03591-y
pmc: PMC6790185
doi:
Substances chimiques
Antioxidants
0
Biomarkers
0
Cardiotonic Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
73-80Références
Goswami SK, Maulik N, Das DK (2007) Ischemia-reperfusion and cardioprotection: a delicate balance between reactive oxygen species generation and redox homeostasis. Ann Med 39:275–289. https://doi.org/10.1080/07853890701374677
doi: 10.1080/07853890701374677
pubmed: 17558599
Zhou T, Chuang C-C, Zuo L (2015) Molecular characterization of reactive oxygen species in myocardial ischemia-reperfusion injury. Biomed Res Int 2015:1–9. https://doi.org/10.1155/2015/864946
doi: 10.1155/2015/864946
Han J, Wang D, Yu B, Wang Y, Ren H, Zhang B, Wang Y, Zheng Q (2014) Cardioprotection against ischemia/reperfusion by licochalcone B in isolated rat hearts. Oxid Med Cell Longev 2014:1–11. https://doi.org/10.1155/2014/134862
doi: 10.1155/2014/134862
Rjiba-Touati K, Ayed-Boussema I, Guedri Y, Achour A, Bacha H, Abid-Essefi S (2015) Effect of recombinant human erythropoietin on mitomycin C-induced oxidative stress and genotoxicity in rat kidney and heart tissues. Hum Exp Toxicol 35:53–62. https://doi.org/10.1177/0960327115577521
doi: 10.1177/0960327115577521
pubmed: 25733728
Liu S, He L, Yao K (2018) The antioxidative function of alpha-ketoglutarate and its applications. Biomed Res Int 2018:3408467. https://doi.org/10.1155/2018/3408467
doi: 10.1155/2018/3408467
pubmed: 29750149
pmcid: 5884300
Zdzisińska B, Żurek A, Kandefer-Szerszeń M (2017) Alpha-ketoglutarate as a molecule with pleiotropic activity: well-known and novel possibilities of therapeutic use. Arch Immunol Ther Exp 65:21–36. https://doi.org/10.1007/s00005-016-0406-x
doi: 10.1007/s00005-016-0406-x
Shapla UM, Solayman M, Alam N, Khalil MI, Gan SH (2018) 5-Hydroxymethylfurfural (HMF) levels in honey and other food products: effects on bees and human health. Chem Cent J 12:35. https://doi.org/10.1186/s13065-018-0408-3
doi: 10.1186/s13065-018-0408-3
pubmed: 29619623
pmcid: 5884753
Zhao L, Chen J, Su J, Li L, Hu S, Li B, Zhang X, Xu Z, Chen T (2013) In vitro antioxidant and antiproliferative activities of 5-hydroxymethylfurfural. J Agric Food Chem 61:10604–10611. https://doi.org/10.1021/jf403098y
doi: 10.1021/jf403098y
pubmed: 24107143
Zolali E, Hamishehkar H, Maleki-Dizaji N, Majidi Zolbanin N, Ghavimi H, Kouhsoltani M, Asgharian P (2014) Selenium effect on oxidative stress factors in septic rats. Adv Pharm Bull 4:289–293. https://doi.org/10.5681/apb.2014.042
doi: 10.5681/apb.2014.042
pubmed: 24754014
pmcid: 3992966
Duntas LH, Benvenga S (2015) Selenium: an element for life. Endocrine 48:756–775. https://doi.org/10.1007/s12020-014-0477-6
doi: 10.1007/s12020-014-0477-6
pubmed: 25519493
Martínez Y, Li X, Liu G, Bin P, Yan W, Más D, Valdivié M, Hu C-AA, Ren W, Yin Y (2017) The role of methionine on metabolism, oxidative stress, and diseases. Amino Acids 49:2091–2098. https://doi.org/10.1007/s00726-017-2494-2
doi: 10.1007/s00726-017-2494-2
pubmed: 28929442
Colovic MB, Vasic VM, Djuric DM, Krstic DZ (2018) Sulphur-containing amino acids: protective role against free radicals and heavy metals. Curr Med Chem 25:324–335. https://doi.org/10.2174/0929867324666170609075434
doi: 10.2174/0929867324666170609075434
pubmed: 28595554
Schwarz M, Greilberger J, Lamacie M, Wasler A, Wintersteiger R, Lang I, Santos RAS (2012) Alpha-ketoglutarate, 5-hydroxy-methylfurfurale and Angiotensin 1-7: cardioprotective effects during myocardial ischemia/reperfusion. Can J Cardiol 28:132. https://doi.org/10.1016/j.cjca.2012.07.123
doi: 10.1016/j.cjca.2012.07.123
Pachauri P, Garabadu D, Goyal A, Upadhyay PK (2017) Angiotensin (1-7) facilitates cardioprotection of ischemic preconditioning on ischemia-reperfusion-challenged rat heart. Mol Cell Biochem 430:99–113. https://doi.org/10.1007/s11010-017-2958-4
doi: 10.1007/s11010-017-2958-4
pubmed: 28293875
Ferreira AJ, Santos RAS, Almeida AP (2002) Angiotensin-(1-7) improves the post-ischemic function in isolated perfused rat hearts. Braz J Med Biol Res 35:1083–1090. https://doi.org/10.1590/S0100-879X2002000900009
doi: 10.1590/S0100-879X2002000900009
pubmed: 12219180
Ferreira AJ, Santos RAS, Almeida AP (2001) Angiotensin-(1-7): cardioprotective effect in myocardial ischemia/reperfusion. Hypertension 38:665–668. https://doi.org/10.1161/01.HYP.38.3.665
doi: 10.1161/01.HYP.38.3.665
pubmed: 11566952
Marrocco I, Altieri F, Peluso I (2017) Measurement and clinical significance of biomarkers of oxidative stress in humans. Oxid Med Cell Longev 2017:6501046. https://doi.org/10.1155/2017/6501046
doi: 10.1155/2017/6501046
pubmed: 28698768
pmcid: 5494111
Russ M, Weinheimer L, Jauk S, Andrä M, Wintersteiger R, Ortner A (2019) Characterization of oxidative stress in chicken heart tissue by high-performance liquid chromatography with ultraviolet-visible (HPLC–UV–Vis) detection. Anal Lett 52:892–901. https://doi.org/10.1080/00032719.2018.1505899
doi: 10.1080/00032719.2018.1505899
Li YX, Li Y, Qian ZJ, Kim MM, Kim SK (2009) In vitro antioxidant activity of 5-HMF isolated from marine red alga Laurencia undulata in free-radical-mediated oxidative systems. J Microbiol Biotechn 19:1319–1327. https://doi.org/10.4014/jmb.0901.0004
doi: 10.4014/jmb.0901.0004
Li W, Qu X-N, Han Y, Zheng S-W, Wang J, Wang Y-P (2015) Ameliorative effects of 5-hydroxymethyl-2-furfural (5-HMF) from Schisandra chinensis on alcoholic liver oxidative injury in mice. Int J Mol Sci 16:2446–2457. https://doi.org/10.3390/ijms16022446
doi: 10.3390/ijms16022446
pubmed: 25622257
pmcid: 4346845
Han X-Y, Hu J-N, Wang Z, Wei S-N, Zheng S-W, Wang Y-P, Li W (2017) 5-HMF attenuates liver fibrosis in CCl4-plus-alcohol-induced mice by suppression of oxidative stress. J Nutr Sci Vitaminol 63:35–43. https://doi.org/10.3177/jnsv.63.35
doi: 10.3177/jnsv.63.35
pubmed: 28367924
Bayliak MM, Lylyk MP, Vytvytska OM, Lushchak VI (2016) Assessment of antioxidant properties of alpha-keto acids in vitro and in vivo. Eur Food Res Technol 242:179–188. https://doi.org/10.1007/s00217-015-2529-4
doi: 10.1007/s00217-015-2529-4
Bayliak MM, Shmihel HV, Lylyk MP, Vytvytska OM, Storey JM, Storey KB, Lushchak VI (2015) Alpha-ketoglutarate attenuates toxic effects of sodium nitroprusside and hydrogen peroxide in Drosophila melanogaster. Environ Toxicol Pharmacol 40:650–659. https://doi.org/10.1016/j.etap.2015.08.016
doi: 10.1016/j.etap.2015.08.016
pubmed: 26363988